About: Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells

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About: Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.virusres.2011.05.005
Description	In most cells, vaccinia virus (VACV) infection is considered to cause a lytic cell death, an equivalent of necrosis. In this paper, we have further analyzed the type of cell death in VACV-infected cells HeLa G and BSC-40. In a cell-based flow cytometric assay, we showed a specific activation of caspase-2 and 4 in HeLa G and BSC-40 cells infected with VACV, strain WR. The actual activity of the two caspases, but also of caspase-3, was then confirmed in lysates of infected HeLa G, but not in BSC-40 cells. PARP cleavage, as well as morphological features of apoptosis and/or activity of the executioner caspase-3 in situ were found increased only in infected HeLa G cells. Finally, vaccination strains Dryvax and Praha were found to induce apoptosis in both HeLa G and BSC-40 cells, as characterized morphologically and by PARP cleavage. These findings may be important for understanding the differences in VACV-host interactions and post-vaccination complications in different individuals. In most cells, vaccinia virus (VACV) infection is considered to cause a lytic cell death, an equivalent of necrosis. In this paper, we have further analyzed the type of cell death in VACV-infected cells HeLa G and BSC-40. In a cell-based flow cytometric assay, we showed a specific activation of caspase-2 and 4 in HeLa G and BSC-40 cells infected with VACV, strain WR. The actual activity of the two caspases, but also of caspase-3, was then confirmed in lysates of infected HeLa G, but not in BSC-40 cells. PARP cleavage, as well as morphological features of apoptosis and/or activity of the executioner caspase-3 in situ were found increased only in infected HeLa G cells. Finally, vaccination strains Dryvax and Praha were found to induce apoptosis in both HeLa G and BSC-40 cells, as characterized morphologically and by PARP cleavage. These findings may be important for understanding the differences in VACV-host interactions and post-vaccination complications in different individuals. (en)
Title	Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells (en)
skos:prefLabel	Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells (en)
skos:notation	RIV/00216208:11110/11:8927!RIV12-GA0-11110___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA310/05/0477), P(GD310/05/H533), S, Z(MSM0021620806)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Mělková, Zora Lišková, Jana Honner, Richard Knitlová, Jarmila
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	186712
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/11:8927
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	vaccinia virus; western reserve; Dryvax; apoptosis; caspases (en)
http://linked.open.../riv/klicoveSlovo	Dryvax caspases vaccinia virus western reserve apoptosis
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[505005682FB2]
http://linked.open...i/riv/nazevZdroje	Virus Research
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	VACCINIA VIRUS INFECTION: TYPE OF HOST-CELL DEATH, CHANGES OF ITS METABOLISM AND REDOX POTENTIAL Study of infectious diseases and their causative agents
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	160
http://linked.open...iv/tvurceVysledku	Honner, Richard Knitlová, Jarmila Lišková, Jana Mělková, Zora
http://linked.open...ain/vavai/riv/wos	000295345600005
http://linked.open...n/vavai/riv/zamer	Molekulární biologie a patologie buňky za normy a u vybraných klinicky závažných patologických procesů
issn	0168-1702
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	11110

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