About: Functional Genomics of Dioecious Plants     Goto   Sponge   NotDistinct   Permalink

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  • Cílem projektu je strukturní a funkční analýza pohlavně vázaných genů u modelových dvoudomých rostlin Silene latifolia a Rumex acetosa. K dosažení tohoto cíle je mj. nutné zvládnout nové technologie genetické modulace studovaných genů. Během tří let plánovaného projektu budou identifikovány geny vázané na pohlavní chromosomy, vyvinuta metodika pro cílenou mutagenezi a umlčování studovaných genů. Tento projekt by měl zásadně přispět k objasnění funkce pohlavních drah u modelových dvoudomých rostlin.
  • Techniques of massive sequencing have recently enabled to identify thousands of genes in various organisms. However, their functional validation remains rather complicated especially in organisms, in which techniques of genetic transformation and/or targeted mutagenesis are not feasible. In model dioecious plants – white campion (Silene latifolia) and sorrel (Rumex acetosa) – possessing evolutionary young sex chromosomes first sex-linked genes have been recently found. These genes are important clues to solve the complex process of sex separation and evolution of sex chromosomes. The project is focused on identification and functional analysis of sex-linked genes and genes with sex-specific expression. An efficient method enabling targeted mutagenesis and silencing of sex-linked genes will be developed. Selected sex-linked genes will be switched-off and their phenotype studied. This project has already been started as a result of successful collaboration between the laboratory of the Czech applicant and the American partner that belongs among the world-class pioneers of targeted mutagenesis in plants and other organisms. (en)
Title
  • Functional Genomics of Dioecious Plants (en)
  • Funkční genomika dvoudomých rostlin
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  • LH14002
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  • functional genomics; gene targeting; nucleases; amiRNA; plant transgenosis; Silene latifolia; Rumex acetosa; sex-linked genes; sex chromosomes (en)
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  • functional genomics
  • Rumex acetosa
  • Silene latifolia
  • amiRNA
  • gene targeting
  • nucleases
  • plant transgenosis
  • sex-linked genes
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