About: Cascade chemoenzymatic reactions of nitriles     Goto   Sponge   NotDistinct   Permalink

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  • Kombinací (bio)katalytických kroků provést v laboratorních podmínkách transformace nitrilů vedoucí ke sloučeninám průmyslového významu nebo využitelné pro screening enzymů. (cs)
  • Cascade reactions represent the current trend in biocatalysis as means to improve its economic and ecological viability (due to no need for intermediate isolation, better product purity, racemization of substrates or regeneration of cofactor in situ). The project aim is to examine the possibilities of multistep catalysis in nitrile biotransformations leading to high-value carboxylic acids (nicotinic, isonicotinic, mandelic, lysergic etc.) and hydroxamic acids (chelators). The action of nitrilases and amidases will be integrated to produce high-purity carboxylic acids from nitriles (the role of amidase being hydrolysis of the nitrilase by-products, amides). Nitrile hydratases (or mild chemical catalysts) and amidases from different strains will be combined to give carboxylic acids from nitriles, if enzymes produced by single strain are not compatible in terms of substrate specificity. The same enzymes will be used to afford hydroxamic acids by carrying out the amidase-catalyzed reaction in presence of (en)
Title
  • Cascade chemoenzymatic reactions of nitriles (en)
  • Kaskádové chemoenzymatické reakce nitrilů (cs)
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  • biocatalysis; biotransformation; cascade reactions; nitriles; amides; carboxylic acids; hydroxamic acids; nitrilase; nitrile hydratase; amidase (en)
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