The major aim of the project is the elucidation of molecular mechanisms underlying the therapeutic effectiveness of compounds isolated from five selected Chinese medicinal herbs with recorded anti-inflammatory and immunomodulatory effects. The subject will be carried out through the bioassay guided fractionation of raw samples leading finally to isolation and identification of pure chemical entities. Several parameters of stimulation or inhibition of the activity of immunocompetent cells will be evaluated: production of nitric oxide, secretion of cytokines/chemokines, production of prostaglandins, and cytotoxic effects. The ultimate goal of the study is discovery of new biological response modifiers, and formulation of novel immunomodulatory drugs prospective in the treatment of immune-related diseases. (en)
Cílem je získat poznatky o imunobiologických vlastnostech látek přírodního původu. Budou analyzovány jejich stimulační nebo inhibiční účinky na produkci oxidu dusnatého, cytokinů, prostaglandinů, a cytotoxicita. Konečným cílem je vývoj nových imunofarmak (cs)