About: Exocyst complex functions in plant cytoplasmic membrane proteins recycling and auxin and brassinosteroid signaling     Goto   Sponge   NotDistinct   Permalink

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  • We propose to continue fruitful collaboration, which was supported by the MSMT KONTAKT programme (proj. num. ME841) from 2006 to the present. This collaboration resulted in the discovery and initial characterization of the plant exocyst, a vesicle tethering complex which is an important regulator of eukaryotic cell morphogenesis (Hala, Cole et al. 2008, Cole et al. 2005, Zarsky and Fowler 2009). For the Prague lab, we propose to concentrate further on the detailed characterization of the Exo70 subunits of exocyst, especially Exo70A1 using also a loss of function mutant of the Arabidopsis exo70A1 (Synek et al. 2006) and some other selected exocyst subunits. We have recently performed a transcriptomic analysis of the exo70A1 mutant (data not published), and have initiated a functional cellular analysis with respect to Exo70A1’s involvement in cytoplasmic membrane dynamics and polarization. Our preliminary data imply that Exo70A1 regulates the recycling of specific plasma membrane proteins, and one as (en)
  • Cílem projektu je porozumění funkci podjednotky Exo70 komplexu exocyst u rostlin ve vztahu k účasti na recyklaci a polarizaci bílkovin plasmalemy. Důraz pak bude na analýza podílu této podjednotky exocystu na řízení polárního transportu auxinu. (cs)
Title
  • Funkce komplexu exocyst v recyklaci bílkovin cytoplasmatické membrány rostlin a auxinové a brassinosteroidové signalizaci (cs)
  • Exocyst complex functions in plant cytoplasmic membrane proteins recycling and auxin and brassinosteroid signaling (en)
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  • plant cell polarity; plant cell morphogenesis; secretion; vesicular membrane trafficking; tethering complexes; exocyst; exocyst subunits; Exo70; Sec8; auxin; brassinosteroids (en)
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