About: Effect of drought and heat stress on polyamine metabolism and contents of phenolics, auxin and abscisic acid in Norway spruce somatic embryos.     Goto   Sponge   NotDistinct   Permalink

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  • Cílem předkládaného projektu je charakteristika stresové odpovědi somatických embryí smrku na působení sucha, vysoké teploty a UV-B záření. Soustředíme se na stanovení obsahu polyaminů a aktivit odpovídajících biosyntetických enzymů, obsahů fenolických kyselin, kyseliny abscisové, auxinu a stilbenů v reakci na stresové podmínky sucha a zvýšené teploty. Jako marker lipidové peroxidace membrán budou stanoveny hodnoty malondialdehydu (MDA). Biochemické analýzy doplní histochemické testy na přítomnost polyfenolických látek s UV-B ochranným charakterem na řezech somatickými embryi po ozáření. (cs)
  • The aim of the project is to evaluate the alterations in polyamine metabolism and in the levels of hydroxycinnamic acids, flavonoids and stilbenes in the stress response of somatic embryos of Norway spruce to drought and high temperature. The determined contents of abscisic acid and auxin will elucidate the abscisic acid-auxin cross-talk in the response to stress. The extent of lipid peroxidation (as indicated by MDA levels) in stressed somatic embryos will be also investigated. Histochemical tests for the presence of polyphenolic substances on embryo sections after the exposure of somatic embryos to UV-B will complete the biochemical analyses. The obtained results will contribute to understanding of the function of these protective elements in plant abiotic stress tolerance. (en)
Title
  • Effect of drought and heat stress on polyamine metabolism and contents of phenolics, auxin and abscisic acid in Norway spruce somatic embryos. (en)
  • Vliv sucha a zvýšené teploty na metabolismus polyaminů a obsahy fenolů, auxinu a kyseliny abscisové v somatických embryích smrku. (cs)
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  • abiotic stres, somatic embryos of Norway spruce, polyamines, phenolic substances, auxin, abscisic acid, histochemical tests (en)
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