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  • Grantově projekt je zamŘýen na vězkum specifickěch vliv… dvojmocněch iont… na strukturnˇ a hybridizaźnˇ vlastnosti ýetŘzc… nukleověch kyselin a jejich syntetickěch analog. Hlavnˇ pozornost bude vŘnov na kr tkěm nukleotid…m, kter‚ slou§ˇ jako molekul rnˇ modely pro testov nˇ vhodnosti modifikace internukleotidov‚ vazby z hlediska potenci lnˇho chemoterapeutick‚ho vyu§itˇ. Materi lovŘ je tento post-doktorandskě projekt sv z n s nosněm projektem v r mci nŘho§ budou nukleotidov  analoga pýipravov na. Hlavnˇmetodou ýeçenˇ projektu bude Ramanova spektroskopie, doplnŘn  podle potýeby o dalçˇ spektroskopick‚ techniky. Bude aplikov na metodika spektroskopick‚ titrace a mŘýenˇ teplotnˇch z vislostˇ spekter. DoplĺkovŘ budou prov dŘna mŘýenˇ polarizovan‚ho Ramanovrozptylu na referenźnˇch molekul rnˇch krystalech relevantnˇch nukleotid… a jejich komplex…. (cs)
  • The grant project is devoted to the study on specific impact of divalent ions on the structural and hybridization properties of nucleic acids and their synthetic analogues. Main attention will be paid to short nucleotides, which serve as molecular modelsin testing of the internucleotide linkage modification suitability from the point of view of potential chemotherapeutic utilization. This post-doc project is attached to the main project within the framework of which the nucleotide analogues will be prepared. The experimental approach will be based on Raman spectroscopy, completed by other spectroscopic techniques whenever necessary. Methods of spectroscopic titration and temperature dependent spectral measurements will be employed. In addition, complementary polarized Raman investigations of reference molecular single microcrystals of relevant nucleotides and their analogues will be carried out. (en)
Title
  • Influence of divalent cations on binding and structural properties of short nucleotides and their analogues (en)
  • Vliv dvojmocněch kationt… na vazebn‚ a strukturnˇ vlastnosti kr tkěch nukleotid… a jejich analog (cs)
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