About: Construction of BAC DNA libraries specific for chromosome 4AL, and positional cloning of gene for adult plant resistance to powdery mildew in wheat     Goto   Sponge   NotDistinct   Permalink

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  • Infection of wheat by fungus B. graminis is causing powdery mildew disease. Recently, non-race-specific powdery mildew resistance gene QPm.tut-4A was mapped on distal end of long arm of wheat chromosome 4A. Here we propose to construct two BAC DNA libraries specific for 4AL chromosomal arm. The huge genome and polyploid character hamper positional cloning in wheat. The proposed libraries would become keys for solving the obstruction and stepping stones for creation of physical map of the 4A chromosome. The physical map will facilitate cloning of important genes from the chromosome, including resistance genes to powdery mildew, leaf rust, Hessian fly, yellow rust, stem rust, wheat streak mosaic and Septoria tritici blotch. The main objective of the project is cloning of the powdery mildew resistance gene QPm.tut-4A. High density map of the QPm.tut-4A gene region will be created and BAC contig spanning the region identified. Subsequently, a pooled genomic BAC library will be prepared from the donor of resistance, T. militinae, and will be used to clone the QPm.tut-4A gene. (en)
  • Cílem navrhovaného projektu je konstrukce BAC DNA knihoven z dlouhého ramene pšeničného chromosomu 4A pro přípravu vysoko-hustotní a fyzické mapy regionu genu QPm.tut-4A odpovědného za zvýšenou rezistenci k padlí travní a klonování tohoto genu. (cs)
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  • Construction of BAC DNA libraries specific for chromosome 4AL, and positional cloning of gene for adult plant resistance to powdery mildew in wheat (en)
  • Konstrukce BAC knihoven specifických pro chromosom 4AL a poziční klonování genu pro rezistenci dospělých rostlin k padlí travní u pšenice (cs)
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  • Triticum aestivum; BAC; chromozómově specifické knihovny, poziční klonování; padlí travní (en)
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