About: The role of tick alpha-2-macroglobulin in pathogen transmission by the hard tick Ixodes ricinus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: The role of tick alpha-2-macroglobulin in pathogen transmission by the hard tick Ixodes ricinus Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
Description	In this project we intend to prove that tick alpha-2-macroglobulin (IrAM) mediates the pathogen transmission by the hard tick Ixodes ricinus. We will study the interaction of IrAM with two tick pathogens: a model Gram-negative bacteria Chryseobacterium indologenes and the Lyme disease spirochetes Borrelia burgdorferi. The antibodies raised against a recombinant fragment of IrAM will be used to visualize binding of IrAM to both pathogens by immunofluoerscent microscopy. Suitable in vitro and in vivo assays will be designed to ascertain how IrAM inactivation (by methylamin) or silencing of its gene (by dsRNA inteference) will influence the ability of tick hemocytes to phagocytose bacteria and spirochetes. Since IrAM is a broad spectrum protease inhibitor, we will investigate its interaction with proteases associated with pathogen surface. The entire sequence of IrAM will be determined and its structure compared to the homolog protein from the soft tick we have characterized earlier. (en) V tomto projektu hodláme prokázat, že klíštěcí alfa-2-makroglobulin (IrAM) ovlivňuje přenos patogenů klíštětem Ixodes ricinus. Budeme studovat interakci IrAM se dvěmi klíštěcími patogeny: modelovou Gram-negativní bakterií Chryseobacterium indologenes a spirochetou Lymské boreliózy Borrelia burgdorferi. Vizualizace vazby IrAM na oba patogeny bude provedena imunofluorescenční mikroskopií pomocí protilátek připravených proti rekombinantnímu fragmentu IrAM. Budou navrženy vhodné in vitro a in vivo eseje pro zjištění, jak inaktivace IrAM (methylaminem) nebo umlčení jeho genu (dsRNA interfencí) ovlivňuje schopnost klíštěcích hemocytů fagocytovat bakterie a spirochety. IrAM je širokospektrý inhibitor proteáz, a proto budeme zkoumat jeho interakci s proteázami asociovanými s povrchem patogenů. Určíme celou sekvenci IrAM a porovnáme jeho strukturu s homologickým proteinem z klíšťáka, který jsme charakterizovali dříve. (cs)
Title	Úloha klíštěcího alfa-2-makroglobulinu při přenosu patogenů klíštětem Ixodes ricinus (cs) The role of tick alpha-2-macroglobulin in pathogen transmission by the hard tick Ixodes ricinus (en)
http://linked.open...vai/cislo-smlouvy	http://linked.opendata.cz/resource/domain/vavai/cep/smlouva/IAA600220603
http://linked.open...lsi-vedlejsi-obor	http://linked.opendata.cz/resource/domain/vavai/cep/obor/EB
http://linked.open...avai/druh-souteze	http://linked.opendata.cz/resource/domain/vavai/cep/druh-souteze/VS
http://linked.open...domain/vavai/faze	http://linked.opendata.cz/resource/domain/vavai/cep/faze/36413423
http://linked.open...vavai/hlavni-obor	http://linked.opendata.cz/resource/domain/vavai/cep/obor/EG
http://linked.open...vai/vedlejsi-obor	http://linked.opendata.cz/resource/domain/vavai/cep/obor/EC
http://linked.open...vavai/id-aktivity	http://linked.opendata.cz/resource/domain/vavai/cep/aktivita/IA
http://linked.open.../vavai/id-souteze	http://linked.opendata.cz/resource/domain/vavai/cep/soutez/SAV02006-A
http://linked.open...n/vavai/kategorie	http://linked.opendata.cz/resource/domain/vavai/cep/kategorie/1
http://linked.open...vai/klicova-slova	tick, Ixodes ricinus, Lyme disease, alpha-2-macroglobulin, thioester bond, phagocytosis, indirect immunofluorescence (en)
http://linked.open...avai/konec-reseni	2008-12-31 (xsd:date)
http://linked.open...nujicich-prijemcu	0 (xsd:integer)
http://linked.open...avai/poskytovatel	http://linked.opendata.cz/resource/domain/vavai/cep/poskytovatel/AV0
http://linked.open...avai/start-reseni	2006-01-01 (xsd:date)
http://linked.open...ai/statni-podpora	2001 (xsd:integer)
http://linked.open...vavai/typProjektu	http://linked.opendata.cz/resource/domain/vavai/cep/typ/P
http://linked.open...ai/uznane-naklady	2001 (xsd:integer)
http://linked.open...ai/pocet-prijemcu	1 (xsd:integer)
http://linked.open...cet-spoluprijemcu	0 (xsd:integer)
http://linked.open...ai/pocet-vysledku	4 (xsd:integer)
http://linked.open...ku-zverejnovanych	4 (xsd:integer)
is http://linked.open...ain/vavai/projekt of	http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2006/IAA600220603/ico-60077344 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2007/IAA600220603/ico-60077344 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2008/IAA600220603/ico-60077344 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2009/IAA600220603/ico-60077344

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 77 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software