About: Dynamics of Spliceosome assembly     Goto   Sponge   NotDistinct   Permalink

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Description
  • "Během tohoto projektu by měla být zprovozněna metoda ""fluorescence recovery after photobleaching"" (FRAP - obnovení fluorescence po vybělení přesvícením). Následně by tato metoda měla být využita v experimentech zaměřených na získání údajů o relativní mobilitě fluorescenčních fůzních proteinů odpovídajících proteinovým složkám spilceosomu a fluorescentně značených RNA konstruktů nesoucích sekvence nezbytné pro sestavování spliceosomu. Fůzní proteiny budou vneseny v podobě expresních vektorů, RNA budou mikroinjikovány." (cs)
  • During this project the method of fluorescence recovery after photobleaching aided with confocal microscopy should be established in our laboratory. Subsequently the method will be used in experiments determined to yield data on relative mobilities of fluorescent fusion proteins corresponding to protein components of spliceosome and fluorescently labelled RNA constructs bearing specific sequences, that are essential for spliceosome assembly. Fusion proteins will be introduced as expression vectors introduced, RNA constructs by means of microinjection. (en)
Title
  • Dynamics of Spliceosome assembly (en)
  • Dynamika sestavování spliceosomu (cs)
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  • Architecture of the cell nucleus; synthesis and processing of RNA; duplication and repair of DNA (en)
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