About: Polychromatic flow cytometry and regulation of aberrant expression from the perspective of Minimal Residual Disease     Goto   Sponge   NotDistinct   Permalink

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  • We propose to investigate minimal residual disease in all children with acute lymphoblastic and myelogenous leukemia in a referential laboratory, together with diagnostics at the onset of the disease. We will assess the practical value of each subpopulation for MRD study using polychromatic flow cytometry and cell sorting followed by molecular genetic analysis. We will study regulation of and resulting interaction through aberrantly expressed molecules correlating with genotype of leukemia. (en)
  • Navrhujeme vyšetřovat v referenčních laboratořích minimální reziduální nemoc dětí s akutní lymfoblastickou i myeloidní leukémií, spolu s diagnostikou při záchytu onemocnění. Výpovědní hodnotu pro MRN u jednotlivých subpopulací ověříme pomocí mnohobarevné cytometrie a třídění buněk s následnou molekulárně genetickou analýzou. Aberantní expresi molekul korelujících s genotypem budeme studovat po stránce regulace i dopadu na interakci buňky s okolím. (cs)
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  • Polychromatic flow cytometry and regulation of aberrant expression from the perspective of Minimal Residual Disease (en)
  • Mnohobarevná cytometrie a regulace aberantní exprese z pohledu leukemické minimální reziduální nemoci (cs)
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  • leukemia; immunophenotyping; flow cytometry; minimal residual disease; cell sorting; aberrant molecules; molecular biology; epidemiology (en)
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