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  • RNA interference (RNAi) is an evolutionary conserved gene-silencing pathway that can be efficiently utilized as a tool to study gene function. RNAi is initiated by long double-stranded RNAs (dsRNAs), which are processed into small duplexes called small-interfering RNAs (siRNAs). In turn, these duplexes target mRNAs for degradation in a sequence-specific manner. Mouse oocytes, unlike most mammalian cell types, lack an interferon response to long dsRNA. Moreover, they are a rare example of a mammalian cell type with a robust endogenous RNAi pathway. For these reasons microinjection of either long dsRNAs or siRNAs results in efficient, sequence-specific gene silencing. Here, we describe a protocol for preparation and microinjection of long dsRNA into mouse oocytes.
  • RNA interference (RNAi) is an evolutionary conserved gene-silencing pathway that can be efficiently utilized as a tool to study gene function. RNAi is initiated by long double-stranded RNAs (dsRNAs), which are processed into small duplexes called small-interfering RNAs (siRNAs). In turn, these duplexes target mRNAs for degradation in a sequence-specific manner. Mouse oocytes, unlike most mammalian cell types, lack an interferon response to long dsRNA. Moreover, they are a rare example of a mammalian cell type with a robust endogenous RNAi pathway. For these reasons microinjection of either long dsRNAs or siRNAs results in efficient, sequence-specific gene silencing. Here, we describe a protocol for preparation and microinjection of long dsRNA into mouse oocytes. (en)
Title
  • RNAi-based methods for gene silencing in mouse oocytes
  • RNAi-based methods for gene silencing in mouse oocytes (en)
skos:prefLabel
  • RNAi-based methods for gene silencing in mouse oocytes
  • RNAi-based methods for gene silencing in mouse oocytes (en)
skos:notation
  • RIV/68378050:_____/13:00423294!RIV14-AV0-68378050
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  • 103126
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  • RIV/68378050:_____/13:00423294
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  • RNAi; dsRNA; mouse oocyte; microinjection; knockdown (en)
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  • [CED3A720B77E]
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  • New York
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  • Methods in Molecular Biology, 957
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  • Mammalian Oocyte Regulation
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  • Svoboda, Petr
  • Schultz, R. M.
  • Stein, P.
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  • 10.1007/978-1-62703-191-2
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  • Humana Press
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  • 978-1-62703-190-5
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