About: Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract.

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About: Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A strictly anaerobic, mesophilic and chitinolytic bacterial strain was isolated from human feces. Based on morphological and physiological properties and 16S rRNA sequence analysis the strain was identified as Clostridium paraputrificum.The strain utilized chitin and N-acetyl-D-glucosamine, grew on glucose and hydrolyzed starch. Cultivation of the strain with colloidal chitin as the growth substrate resulted in the production of gas and formation of acetate and lactate and only small quantities of propionate and butyrate. In the course of a 10-d cultivation with chitin, the endochitinase activity was detected after 1 d and gradually increased, reaching maximum after 3 d. The beta-N-acetylglucosaminidase activity appeared just at the beginning of the cultivation, increased to day 2 and then remained nearly constant. More than 90 of chitin added was degraded within 2 d of cultivation. On the zymogram of the extracelular chitinolytic complex were visible at least 6 isoenzymes with molar mass 43.5-65.0 k A strictly anaerobic, mesophilic and chitinolytic bacterial strain was isolated from human feces. Based on morphological and physiological properties and 16S rRNA sequence analysis the strain was identified as Clostridium paraputrificum.The strain utilized chitin and N-acetyl-D-glucosamine, grew on glucose and hydrolyzed starch. Cultivation of the strain with colloidal chitin as the growth substrate resulted in the production of gas and formation of acetate and lactate and only small quantities of propionate and butyrate. In the course of a 10-d cultivation with chitin, the endochitinase activity was detected after 1 d and gradually increased, reaching maximum after 3 d. The beta-N-acetylglucosaminidase activity appeared just at the beginning of the cultivation, increased to day 2 and then remained nearly constant. More than 90 of chitin added was degraded within 2 d of cultivation. On the zymogram of the extracelular chitinolytic complex were visible at least 6 isoenzymes with molar mass 43.5-65.0 k (en)
Title	Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract. Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract. (en)
skos:prefLabel	Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract. Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract. (en)
skos:notation	RIV/67985904:_____/02:21023078!RIV/2003/AV0/A21003/N
http://linked.open.../vavai/riv/strany	559;564
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA525/00/0984), P(KSK5052113), Z(AV0Z5045916)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Kopečný, Jan Hodrová, Blanka Šimůnek, Jiří Bartoňová, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	648265
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/02:21023078
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Clostridium paraputrificum; Chitinolytic bacterium; digestive tract (en)
http://linked.open.../riv/klicoveSlovo	digestive tract Chitinolytic bacterium Clostridium paraputrificum
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[518E827B3008]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Structure, expression and interaction of the genome The effect of dietary chitin on microbes in the digestive tract and the decrease of hypercholesterolemia
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	47
http://linked.open...iv/tvurceVysledku	Kopečný, Jan Šimůnek, Jiří Bartoňová, Hana Hodrová, Blanka
http://linked.open...n/vavai/riv/zamer	Základní výzkum genetických struktur, fyziologických funkcí a vývojové biologie obratlovců se zaměřením na biomedicínsky, hospodářsky i jinak významné druhy
issn	0015-5632
number of pages	5 (xsd:int)

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