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  • Předneseno na:HAKONEXIV,Greifswald,Inactivation of Escherichia coli using non-thermal surface dielectric barrier discharges (SDBD’s) was widely studied; however, the complete mechanism of plasma-assisted inactivation is still not known.We investigated processes involved in the plasma-assisted inactivation with a particular emphasis on how microorganisms response to plasma-induced stress.The single streamer filament SDBD was produced in coplanar electrode geometry and driven by amplitude-modulated AC high voltage waveforms in humid synthetic air at atmospheric pressure.The response to plasma-induced stress was estimated by conventional cultivation,sub-lethal injury assay, resazurin assay and LIVE/DEAD BacLight BacterialViability kit. Treated bacteria partly lost ability to grow precisely and became injured and cultivable or even morphologically intact(viable/active) but non-culturable (VBNC/ABNC).Formation of damaged membranes was observed through propidium iodide,which labels only
  • Předneseno na:HAKONEXIV,Greifswald,Inactivation of Escherichia coli using non-thermal surface dielectric barrier discharges (SDBD’s) was widely studied; however, the complete mechanism of plasma-assisted inactivation is still not known.We investigated processes involved in the plasma-assisted inactivation with a particular emphasis on how microorganisms response to plasma-induced stress.The single streamer filament SDBD was produced in coplanar electrode geometry and driven by amplitude-modulated AC high voltage waveforms in humid synthetic air at atmospheric pressure.The response to plasma-induced stress was estimated by conventional cultivation,sub-lethal injury assay, resazurin assay and LIVE/DEAD BacLight BacterialViability kit. Treated bacteria partly lost ability to grow precisely and became injured and cultivable or even morphologically intact(viable/active) but non-culturable (VBNC/ABNC).Formation of damaged membranes was observed through propidium iodide,which labels only (en)
Title
  • Inactivation of Escherichia coli by surface streamer discharges(HAKONEXIV)
  • Inactivation of Escherichia coli by surface streamer discharges(HAKONEXIV) (en)
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  • Inactivation of Escherichia coli by surface streamer discharges(HAKONEXIV)
  • Inactivation of Escherichia coli by surface streamer discharges(HAKONEXIV) (en)
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  • RIV/61389021:_____/14:00436700!RIV15-AV0-61389021
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  • RIV/61389021:_____/14:00436700
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  • Non-thermal surface dielectric barrier discharge; bacterial decontamination; viable; but non-culturable (en)
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  • Šimek, Milan
  • Doležalová, Eva
  • Prukner, Václav
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