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  • Insufficiency of information on the factors controlling the growth and metabolic capabilities of groundwater bacteria still limits successful implementation of enhanced bioremediation technologies. Genome of P. fluorescens ICT was analysed by 454-pyrosequencing technology. In total, 121 Mb were obtained by two sequencing runs on GS Junior System. The assembly of 454-pyrosequencing data resulted in a construction of 73 contigs with an average length of 99,068 bps. MG-RAST and Blast2Go were used for gene annotations. The predicted size of the genome was 7.2 MB. Of the total genome sequence, 97.2% were proteins annotated by MG-RAST, 91.2% of annotated features were assigned to functional categories. Tested laboratory strain was found to be closely related to the P. fluorescens Pf-5 (Accession No. CP000076) genome with 96% of genes with E below 10-30. Among others, the bacterium contains 18 genes involved in biphenyl metabolism, 10 involved in the metabolism of chlorinated aromatic compounds and 9 genes for naphthalene and anthracene metabolism.
  • Insufficiency of information on the factors controlling the growth and metabolic capabilities of groundwater bacteria still limits successful implementation of enhanced bioremediation technologies. Genome of P. fluorescens ICT was analysed by 454-pyrosequencing technology. In total, 121 Mb were obtained by two sequencing runs on GS Junior System. The assembly of 454-pyrosequencing data resulted in a construction of 73 contigs with an average length of 99,068 bps. MG-RAST and Blast2Go were used for gene annotations. The predicted size of the genome was 7.2 MB. Of the total genome sequence, 97.2% were proteins annotated by MG-RAST, 91.2% of annotated features were assigned to functional categories. Tested laboratory strain was found to be closely related to the P. fluorescens Pf-5 (Accession No. CP000076) genome with 96% of genes with E below 10-30. Among others, the bacterium contains 18 genes involved in biphenyl metabolism, 10 involved in the metabolism of chlorinated aromatic compounds and 9 genes for naphthalene and anthracene metabolism. (en)
Title
  • Metabolic pathways of Pseudomonas fluorescens ICT in the perspective of groundwater remediation
  • Metabolic pathways of Pseudomonas fluorescens ICT in the perspective of groundwater remediation (en)
skos:prefLabel
  • Metabolic pathways of Pseudomonas fluorescens ICT in the perspective of groundwater remediation
  • Metabolic pathways of Pseudomonas fluorescens ICT in the perspective of groundwater remediation (en)
skos:notation
  • RIV/46747885:24620/13:#0000379!RIV14-MPO-24620___
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  • P(FR-TI4/278)
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  • 87459
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  • RIV/46747885:24620/13:#0000379
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  • Pseudomonas fluorescens, bioremediation, metabolic pathways, genome sequencing (en)
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  • [DF20DC3BEE1E]
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  • Ševců, Alena
  • Sakmaryová, Iva
  • Baldrian, P.
  • Chroňáková, A.
http://localhost/t...ganizacniJednotka
  • 24620
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