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| rdfs:seeAlso
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| Description
| - Little cherry disease (LChD), a virus disease of sweet (Prunus avium) and sour cherries (P. cerasus), is caused by members of the Closteroviridae family. Symptoms are especially visible on fruits and leaves. Leaves become red or bronze in late summer. Fruit are small, angular, pointed and unmarketable due to a characteristic bitter flavor. LChD also causes reduction of yield (1). The presence of LChD was determined in orchards in the East Bohemia Region of the Czech Republic by reverse transcription (RT)-PCR and woody indicator plants, as recommended by the European and Mediterranean Plant Protection Organization (EPPO). Total RNA was isolated with a CONCERT Plant RNA Purification Reagent (Invitrogen, Carlsbad, CA) (3). RT-PCR was performed with a QIAGEN OneStep RT-PCR Kit (Qiagen, Hilden, Germany) and oligonucleotides previously described (4). Oligonucleotide LCV3EC (5'-GCTCTAGAGGCACCTTTTATTTTTTATATATGC-3') was used as a negative-sense primer, oligonucleotide LCV16659 (5'-GTTATAGAATTCACTGCAAGTG-3') was used as a positive-sense primer in RT reactions and PCR amplification. The PCR products (430 bp) were analyzed on 1% agarose gels (stained with SYBR green). According to the preliminary results, young leaves from buds (67% of samples of selected trees with LChD were positive), blossoms (67% positive), and leaves taken in autumn (67% positive) were optimal for the detection of LChD by RT-PCR. The trial with woody indicator plant species was established in the field. Indicators P. avium cv. Sam and P. avium cvs. Bing, F12/1, and Canindex (4) were inoculated with buds from LChD-infected trees and observed for 2 years. P. avium cv. Canindex seems to be the best woody indicator for testing of LChD in the climatic conditions of the Czech Republic. To our knowledge, this is the first report of LChD in the Czech Republic.
- Little cherry disease (LChD), a virus disease of sweet (Prunus avium) and sour cherries (P. cerasus), is caused by members of the Closteroviridae family. Symptoms are especially visible on fruits and leaves. Leaves become red or bronze in late summer. Fruit are small, angular, pointed and unmarketable due to a characteristic bitter flavor. LChD also causes reduction of yield (1). The presence of LChD was determined in orchards in the East Bohemia Region of the Czech Republic by reverse transcription (RT)-PCR and woody indicator plants, as recommended by the European and Mediterranean Plant Protection Organization (EPPO). Total RNA was isolated with a CONCERT Plant RNA Purification Reagent (Invitrogen, Carlsbad, CA) (3). RT-PCR was performed with a QIAGEN OneStep RT-PCR Kit (Qiagen, Hilden, Germany) and oligonucleotides previously described (4). Oligonucleotide LCV3EC (5'-GCTCTAGAGGCACCTTTTATTTTTTATATATGC-3') was used as a negative-sense primer, oligonucleotide LCV16659 (5'-GTTATAGAATTCACTGCAAGTG-3') was used as a positive-sense primer in RT reactions and PCR amplification. The PCR products (430 bp) were analyzed on 1% agarose gels (stained with SYBR green). According to the preliminary results, young leaves from buds (67% of samples of selected trees with LChD were positive), blossoms (67% positive), and leaves taken in autumn (67% positive) were optimal for the detection of LChD by RT-PCR. The trial with woody indicator plant species was established in the field. Indicators P. avium cv. Sam and P. avium cvs. Bing, F12/1, and Canindex (4) were inoculated with buds from LChD-infected trees and observed for 2 years. P. avium cv. Canindex seems to be the best woody indicator for testing of LChD in the climatic conditions of the Czech Republic. To our knowledge, this is the first report of LChD in the Czech Republic. (en)
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| Title
| - First Report of Little Cherry Disease from Sweet Cherry (Prunus avium) and Sour Cherry (P-cerasus) in the Czech Republic
- First Report of Little Cherry Disease from Sweet Cherry (Prunus avium) and Sour Cherry (P-cerasus) in the Czech Republic (en)
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| skos:prefLabel
| - First Report of Little Cherry Disease from Sweet Cherry (Prunus avium) and Sour Cherry (P-cerasus) in the Czech Republic
- First Report of Little Cherry Disease from Sweet Cherry (Prunus avium) and Sour Cherry (P-cerasus) in the Czech Republic (en)
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| skos:notation
| - RIV/25271121:_____/11:2R002572!RIV12-MZE-25271121
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/25271121:_____/11:2R002572
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Little Cherry Disease; sweet cherry; sour cherry (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Ludvíková, Hana
- Suchá, Jana
|
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