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| Description
| - Aim. To compare aspects of wound healing after cleft lip surgery performed within one week of age and wound healing after surgery performed within 2 - 4 months of age, especially concentrations of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in tissue removed during surgery. Methods. 34 tissue samples (26 boys and 8 girls) were removed during surgery within one week of age (n=19) or within 2 - 4 months of age (n=15). Tissue samples were separated into epidermis, dermis and mucous membrane. Proteins were extracted in cacodylic buffer for 24 h at a temperature 2 - 8 degrees C. Total protein concentrations were examined using a modification of the Lowry method. Samples were examined using ELISA kit Amersham Biotrak Activity Assay (GE Healthcare UK) for detection of MMP-9 and TIMP-1 concentrations. Results. MMP-9: early surgery - epidermis 2.168+/-3.303 mu g/g of protein (mean+/-SD), dermis 1.251+/-1.848 mu g/g, 2 - 4 months surgery - epidermis 0.347+/-0.212 mu g/g, dermis 0.555+/-0.276 mu g/g. TIMP-1: early surgery - epidermis 1.762+/-2.162 mu g/g, dermis 1.628+/-0.822 mu g/g, mucous membrane 2.066+/-1.717 mu g/g, 2 - 4 months surgery - epidermis 1.881+/-2.810 mu g/g, dermis 3.117+/-1.540 mu g/g, mucous membrane 4.833+/-6.550 mu g/g. Conclusions. There were no significant differences in concentrations of protein MMP-9 in epidermis and dermis and TIMP-1 in epidermis and mucous membrane according to time of surgery. Significantly decreased levels of TIMP-1 in dermis were found in samples obtained from early surgery compared to levels in samples obtained from 2 - 4 months surgery.
- Aim. To compare aspects of wound healing after cleft lip surgery performed within one week of age and wound healing after surgery performed within 2 - 4 months of age, especially concentrations of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in tissue removed during surgery. Methods. 34 tissue samples (26 boys and 8 girls) were removed during surgery within one week of age (n=19) or within 2 - 4 months of age (n=15). Tissue samples were separated into epidermis, dermis and mucous membrane. Proteins were extracted in cacodylic buffer for 24 h at a temperature 2 - 8 degrees C. Total protein concentrations were examined using a modification of the Lowry method. Samples were examined using ELISA kit Amersham Biotrak Activity Assay (GE Healthcare UK) for detection of MMP-9 and TIMP-1 concentrations. Results. MMP-9: early surgery - epidermis 2.168+/-3.303 mu g/g of protein (mean+/-SD), dermis 1.251+/-1.848 mu g/g, 2 - 4 months surgery - epidermis 0.347+/-0.212 mu g/g, dermis 0.555+/-0.276 mu g/g. TIMP-1: early surgery - epidermis 1.762+/-2.162 mu g/g, dermis 1.628+/-0.822 mu g/g, mucous membrane 2.066+/-1.717 mu g/g, 2 - 4 months surgery - epidermis 1.881+/-2.810 mu g/g, dermis 3.117+/-1.540 mu g/g, mucous membrane 4.833+/-6.550 mu g/g. Conclusions. There were no significant differences in concentrations of protein MMP-9 in epidermis and dermis and TIMP-1 in epidermis and mucous membrane according to time of surgery. Significantly decreased levels of TIMP-1 in dermis were found in samples obtained from early surgery compared to levels in samples obtained from 2 - 4 months surgery. (en)
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| Title
| - Concentrations of MMP-9 and TIMP-1 in lip tissue and their impact on cleft lip surgery healing
- Concentrations of MMP-9 and TIMP-1 in lip tissue and their impact on cleft lip surgery healing (en)
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| skos:prefLabel
| - Concentrations of MMP-9 and TIMP-1 in lip tissue and their impact on cleft lip surgery healing
- Concentrations of MMP-9 and TIMP-1 in lip tissue and their impact on cleft lip surgery healing (en)
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| skos:notation
| - RIV/00216208:11130/13:10209799!RIV15-MSM-11130___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11130/13:10209799
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - wound healing; cleft lip; matrix metalloproteinases (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Dostálová, Taťjána
- Kašparová, Magdaléna
- Kotaška, Karel
- Pechová, Marta
- Štekláčová, Anna
- Bláha, Karel
- Borsky, Jiří
- Matějová, Radka
- Zajičková, Veronika
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
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| http://localhost/t...ganizacniJednotka
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