About: Expansion of human mesothelial progenitor cells in a long-term three-dimensional organotypic culture of Processus vaginalis peritonei     Goto   Sponge   NotDistinct   Permalink

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Description
  • Autoři popisují, že expanze HMP buněk je dlouhodobou 3D organotypickou kulturou procesus vaginalis peritonei (cs)
  • A 3D culture system was used to investigate the behaviour of mesothelial cells present in the wall of human processus vaginalis peritonei. Small tissue fragments placed on collagen sponges were cultured for 7, 14 and 21 days in medium supplemented with 10% FBS, and analysed for the expression and distribution of cytokeratins (CKAEI-AE3, CK19), p63, Ki-67, vimentin, CD34, and HBNM-1. Before culture, flat mesothelial cells displayed immunoreactivity for cytokeratins, vimentin and HBME-1, while p63 and CD34 were negative. Mesenchymal cells within the stroma were vimentin-positive and endothelial cells of small vessels displayed positive staining for CD34. Cytokeratins, p63 and HBME-1 were negative in all stromal cells in cultured fragments, flat mesothelial cells positive for vimentin, cytokeratins and HBME-1 proliferated, lining the fragment surface and migrating into the sponge. Capillaries showed morphological alterations; however, their immunoreactivity was comparable with the stroma prior to culture
  • A 3D culture system was used to investigate the behaviour of mesothelial cells present in the wall of human processus vaginalis peritonei. Small tissue fragments placed on collagen sponges were cultured for 7, 14 and 21 days in medium supplemented with 10% FBS, and analysed for the expression and distribution of cytokeratins (CKAEI-AE3, CK19), p63, Ki-67, vimentin, CD34, and HBNM-1. Before culture, flat mesothelial cells displayed immunoreactivity for cytokeratins, vimentin and HBME-1, while p63 and CD34 were negative. Mesenchymal cells within the stroma were vimentin-positive and endothelial cells of small vessels displayed positive staining for CD34. Cytokeratins, p63 and HBME-1 were negative in all stromal cells in cultured fragments, flat mesothelial cells positive for vimentin, cytokeratins and HBME-1 proliferated, lining the fragment surface and migrating into the sponge. Capillaries showed morphological alterations; however, their immunoreactivity was comparable with the stroma prior to culture (en)
Title
  • Expansion of human mesothelial progenitor cells in a long-term three-dimensional organotypic culture of Processus vaginalis peritonei
  • Expansion of human mesothelial progenitor cells in a long-term three-dimensional organotypic culture of Processus vaginalis peritonei (en)
  • Expanze HMP buněk je dlouhodobou 3D organotypickou kulturou procesus vaginalis peritonei (cs)
skos:prefLabel
  • Expansion of human mesothelial progenitor cells in a long-term three-dimensional organotypic culture of Processus vaginalis peritonei
  • Expansion of human mesothelial progenitor cells in a long-term three-dimensional organotypic culture of Processus vaginalis peritonei (en)
  • Expanze HMP buněk je dlouhodobou 3D organotypickou kulturou procesus vaginalis peritonei (cs)
skos:notation
  • RIV/00216208:11120/07:00000464!RIV08-MSM-11120___
http://linked.open.../vavai/riv/strany
  • 50-57
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • V
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 421073
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11120/07:00000464
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Processus vaginalis peritonei; mesothelial cells; CD34; cytokeratins; HBME-1 (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [CE0658CC9513]
http://linked.open...i/riv/nazevZdroje
  • Folia Biologica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 53
http://linked.open...iv/tvurceVysledku
  • Mandys, Václav
  • Michelini, M.
  • Rosellini, A.
  • Revoltella, R. P.
  • Tanda, G.
issn
  • 0015-5500
number of pages
http://localhost/t...ganizacniJednotka
  • 11120
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