About: Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis

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About: Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In the current study, the results of an intradermal tuberculin test and a gamma interferon (INF-?) release assay were compared based on the detection of interferon-gamma production after in vitro stimulation with Mycobacterium avium subsp. avium-specific antigen for the discrimination of pigs naturally infected with M. avium subsp. hominissuis. Fifty-five clinically healthy pigs were used in the study. Three of these were proven by culture and real-time quantitative polymerase chain reaction methods to be infected with M. avium subsp. hominissuis (2 animals) and Mycobacterium xenopi (1 animal). No animals were positive by the tuberculin test. Both M. avium subsp. hominissuis-positive pigs were evaluated as positive by the INF-? release assay. Bacteriologically negative and M. xenopi-positive pigs were unresponsive in the INF-? release assay, indicating the specificity of the method. The results suggest that the INF-? release assay has a higher sensitivity than the tuberculin test and that the assay can be used for diagnosis of M. avium infections in live, naturally infected pigs. In the current study, the results of an intradermal tuberculin test and a gamma interferon (INF-?) release assay were compared based on the detection of interferon-gamma production after in vitro stimulation with Mycobacterium avium subsp. avium-specific antigen for the discrimination of pigs naturally infected with M. avium subsp. hominissuis. Fifty-five clinically healthy pigs were used in the study. Three of these were proven by culture and real-time quantitative polymerase chain reaction methods to be infected with M. avium subsp. hominissuis (2 animals) and Mycobacterium xenopi (1 animal). No animals were positive by the tuberculin test. Both M. avium subsp. hominissuis-positive pigs were evaluated as positive by the INF-? release assay. Bacteriologically negative and M. xenopi-positive pigs were unresponsive in the INF-? release assay, indicating the specificity of the method. The results suggest that the INF-? release assay has a higher sensitivity than the tuberculin test and that the assay can be used for diagnosis of M. avium infections in live, naturally infected pigs. (en)
Title	Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis (en)
skos:prefLabel	Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis (en)
skos:notation	RIV/00027162:_____/12:#0000920!RIV13-MZE-00027162
http://linked.open...avai/predkladatel	Výzkumný ústav veterinárního lékařství, v.v.i,
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1B53009), P(ED0006/01/01), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Faldyna, Martin Štěpánová, Hana Slaná, Iva Pavlík, Ivo Kudláčková, Hana Göpfert, Eduard Kaevska, Marija
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	176263
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/12:#0000920
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Gamma interferon; infection; tuberculination (en)
http://linked.open.../riv/klicoveSlovo	infection Gamma interferon tuberculination
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[ADF8E20D7745]
http://linked.open...i/riv/nazevZdroje	Journal of Veterinary Diagnostic Investigation
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine Avian tuberculosis and mycobacterioses: the development of methods for theirs prevention and eradication by study of mycobacterial sources and pathogenesis of infection
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	24
http://linked.open...iv/tvurceVysledku	Faldyna, Martin Kaevska, Marija Pavlík, Ivo Slaná, Iva Štěpánová, Hana Göpfert, Eduard Kudláčková, Hana
http://linked.open...ain/vavai/riv/wos	000307114500021
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	1040-6387
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.1177/1040638711435141
is http://linked.open...avai/riv/vysledek of	Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis

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