About: Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	SYBR Green real-time RT PCR assay was developed and optimized for the sensitive detection of Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), Garlic common latent virus (GCLV), Shallot latent virus (SLV) and Mite-borne filamentous virus (MbFV). The polyvalence of the designed primers was tested on 50 genotypes of garlic (Allium sativum L.) which originated from different countries. Plasmid standards were prepared and used as positive standards. The efficiencies of all reactions were 97, 93, 99, 98 and 87% for OYDV, LYSV, SLV, GCLV and MbFV standards, respectively. The detection limit for OYDV, LYSV and GCLV was as low as five gene copies, for SLV it was 15 gene copies and for MbFV it was 130 gene copies. In comparison with ELISA, more virus-positive garlic accessions were detected with LYSV and GCLV by SYBR Green based real-time RT PCR assay. This method was shown to be a more suitable tool for the detection of highly variable pathogens, such as garlic viruses. SYBR Green real-time RT PCR assay was developed and optimized for the sensitive detection of Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), Garlic common latent virus (GCLV), Shallot latent virus (SLV) and Mite-borne filamentous virus (MbFV). The polyvalence of the designed primers was tested on 50 genotypes of garlic (Allium sativum L.) which originated from different countries. Plasmid standards were prepared and used as positive standards. The efficiencies of all reactions were 97, 93, 99, 98 and 87% for OYDV, LYSV, SLV, GCLV and MbFV standards, respectively. The detection limit for OYDV, LYSV and GCLV was as low as five gene copies, for SLV it was 15 gene copies and for MbFV it was 130 gene copies. In comparison with ELISA, more virus-positive garlic accessions were detected with LYSV and GCLV by SYBR Green based real-time RT PCR assay. This method was shown to be a more suitable tool for the detection of highly variable pathogens, such as garlic viruses. (en)
Title	Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction (en)
skos:prefLabel	Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction (en)
skos:notation	RIV/00027006:_____/11:00001757!RIV12-MZE-00027006
http://linked.open...avai/predkladatel	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QH71228), Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Smékalová, Kateřina Leišová-Svobodová, Leona
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	193707
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/11:00001757
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	OYDV; LYSV; GCLV; SLV; MbFV; SYBR Green real-time RT-PCR (en)
http://linked.open.../riv/klicoveSlovo	GCLV LYSV MbFV OYDV SLV SYBR Green real-time RT-PCR
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[B435363965C2]
http://linked.open...i/riv/nazevZdroje	Journal of Phytopathology
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Optimalization of recovery methods and in vitro propagation for purpose of long-time preservation by cryoconservation of national garlic genotypes
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	159
http://linked.open...iv/tvurceVysledku	Leišová-Svobodová, Leona Smékalová, Kateřina
http://linked.open...ain/vavai/riv/wos	000289626700006
http://linked.open...n/vavai/riv/zamer	Udržitelné systémy pěstování zemědělských plodin pro produkci kvalitních a bezpečných potravin, krmiv a surovin
issn	0931-1785
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1439-0434.2011.01790.x
is http://linked.open...avai/riv/vysledek of	Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction

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