| Attributes | Values |
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| rdf:type
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| Description
| - Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4°C) and short photoperiod (8/16-h – light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4°C/h. The lethal temperature (LT) was -13.0°C for cold hardened and 6.5°C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of c
- Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4°C) and short photoperiod (8/16-h – light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4°C/h. The lethal temperature (LT) was -13.0°C for cold hardened and 6.5°C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of c (en)
- Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4°C) and short photoperiod (8/16-h – light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4°C/h. The lethal temperature (LT) was -13.0°C for cold hardened and 6.5°C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of c (cs)
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| Title
| - Proliferation and cold hardening of in vitro grown apple shoot tips
- Proliferation and cold hardening of in vitro grown apple shoot tips (en)
- Proliferace a mrazové otužování in vitro kultur jabloní (cs)
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| skos:prefLabel
| - Proliferation and cold hardening of in vitro grown apple shoot tips
- Proliferation and cold hardening of in vitro grown apple shoot tips (en)
- Proliferace a mrazové otužování in vitro kultur jabloní (cs)
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| skos:notation
| - RIV/00027006:_____/06:1736!RIV07-MZE-00027006
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/06:1736
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - micropropagation, germplasm, encapsulation, cryopreservation, freezing (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Zámečník, Jiří
- Paprštein, František
- Sedlák, Jiří
- Bilavčík, Alois
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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