About: Sequence analysis of the 5'-terminal part of GLU-D1 locus of landraces and old cultivars of wheat (Triticum aestivum)     Goto   Sponge   NotDistinct   Permalink

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  • We have analyzed 380bp long N-terminal parts of Glu-D1x alleles from several lines of landraces and old cultivars of wheat (Triticum aestivum L.) with the aim to verify, whether alleles characterized as identical according to their mobility in SDS-PAGE are encoded always by the same DNA sequences. European landraces and old cultivars were chosen for the study as a potential source of higher genetic variability as compared with modern wheat cultivars. We have not found any changes in comparison with known data in the sequences of Dx3 and Dx4 alleles, which in fact were rare in the available set of landraces and old cultivars. Three point mutations were found together in Dx2 and Dx5 alleles of landraces and old cultivars, no one in the standard wheat cultivars Chinese Spring and Bezostaya1. Sequencing analysis can be another suitable technique for study of glutenin alleles complementing SDS-PAGE.
  • We have analyzed 380bp long N-terminal parts of Glu-D1x alleles from several lines of landraces and old cultivars of wheat (Triticum aestivum L.) with the aim to verify, whether alleles characterized as identical according to their mobility in SDS-PAGE are encoded always by the same DNA sequences. European landraces and old cultivars were chosen for the study as a potential source of higher genetic variability as compared with modern wheat cultivars. We have not found any changes in comparison with known data in the sequences of Dx3 and Dx4 alleles, which in fact were rare in the available set of landraces and old cultivars. Three point mutations were found together in Dx2 and Dx5 alleles of landraces and old cultivars, no one in the standard wheat cultivars Chinese Spring and Bezostaya1. Sequencing analysis can be another suitable technique for study of glutenin alleles complementing SDS-PAGE. (en)
  • We have analyzed 380bp long N-terminal parts of Glu-D1x alleles from several lines of landraces and old cultivars of wheat (Triticum aestivum L.) with the aim to verify, whether alleles characterized as identical according to their mobility in SDS-PAGE are encoded always by the same DNA sequences. European landraces and old cultivars were chosen for the study as a potential source of higher genetic variability as compared with modern wheat cultivars. We have not found any changes in comparison with known data in the sequences of Dx3 and Dx4 alleles, which in fact were rare in the available set of landraces and old cultivars. Three point mutations were found together in Dx2 and Dx5 alleles of landraces and old cultivars, no one in the standard wheat cultivars Chinese Spring and Bezostaya1. Sequencing analysis can be another suitable technique for study of glutenin alleles complementing SDS-PAGE. (cs)
Title
  • Sekvenční analýza 5'- části GLU-D1 pšenice (cs)
  • Sequence analysis of the 5'-terminal part of GLU-D1 locus of landraces and old cultivars of wheat (Triticum aestivum)
  • Sequence analysis of the 5'-terminal part of GLU-D1 locus of landraces and old cultivars of wheat (Triticum aestivum) (en)
skos:prefLabel
  • Sekvenční analýza 5'- části GLU-D1 pšenice (cs)
  • Sequence analysis of the 5'-terminal part of GLU-D1 locus of landraces and old cultivars of wheat (Triticum aestivum)
  • Sequence analysis of the 5'-terminal part of GLU-D1 locus of landraces and old cultivars of wheat (Triticum aestivum) (en)
skos:notation
  • RIV/00027006:_____/00:107!RIV06-GA0-00027006
http://linked.open.../vavai/riv/strany
  • 414;416
http://linked.open...avai/riv/aktivita
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  • P(GA521/97/0222)
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  • 4
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  • 726242
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  • RIV/00027006:_____/00:107
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http://linked.open.../riv/klicovaSlova
  • Glu-D1 locus, DNA sequence, glutenin, Triticum aestivum, variability (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • HU - Maďarsko
http://linked.open...ontrolniKodProRIV
  • [87F0FE4C2C62]
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  • Cereal Research Communications
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http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...v/svazekPeriodika
  • 28
http://linked.open...iv/tvurceVysledku
  • Ovesná, Jaroslava
issn
  • 0133-3720
number of pages
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