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Description
  • - Prověření možnosti časově rozlišené mikrospektrofluorimetrie pro rozlišení různých vazebných módů porfyrinu v komplexech s oligonukleotidy a aptamery - Spektroskopická studie tvorby komplexu mezi novými kationickými porfyriny a modifikovanými oligonukleotidy - Zpracování a verifikace metodologie založené na dobách života fluoroforu pro kvantitativní vyhodnocení porfyrinem indukovaného vstupu a kvalitativní posouzení integrity oligonukleotidu uvnitř buňky
  • - Test of the applicability of the time-resolved microspectrofluorimetry in order to distinguish various porphyrin binding modes in complexes with oligonucleotides and aptamers. - Spectroscopic study of the complex formation between novel cationic porphyrins and modified oligonucleotides. - Elaboration and verification of the methodology based on the fluorophore lifetimes for quantitative evaluation of the porphyrin-assisted internalization and qualitative assessment of the intracellular oligonucleotide integrity. (en)
Title
  • Time resolved microfluorimetry in application to cellular monitoring of potential chemoterapetics (en)
  • Časově rozlišená mikrospektrofluorimetrie: aplikace pro sledování potenciálních chemoterapeutik v živých buňkách
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  • 7AMB14FR020
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  • Oligonucleotide, aptamer, time-resolved microspectrofluorimetry, cellular uptake (en)
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  • Oligonucleotide
  • aptamer
  • time-resolved microspectrofluorimetry
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