About: Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C. sativus), x = 7; muskmelon (C. melo) and wild Cucumis species, x = 12). Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts. Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C. sativus), x = 7; muskmelon (C. melo) and wild Cucumis species, x = 12). Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts. (en)
Title	Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis (en)
skos:prefLabel	Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis (en)
skos:notation	RIV/61989592:15310/10:10212970!RIV11-MSM-15310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6198959215)
http://linked.open...iv/cisloPeriodika	ID 475432
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Navrátilová, Božena Ondřej, Vladan Skálová, Dagmar Doležalová, Ivana Lebeda, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	279808
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/10:10212970
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	in vitro techniques; Biotechnology; Polyploidization; Cucumis spp. (en)
http://linked.open.../riv/klicoveSlovo	Biotechnology Polyploidization in vitro techniques Cucumis spp.
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[B9A6BC7A702C]
http://linked.open...i/riv/nazevZdroje	Journal of Biomedicine and Biotechnology (online)
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	2010
http://linked.open...iv/tvurceVysledku	Lebeda, Aleš Navrátilová, Božena Ondřej, Vladan Skálová, Dagmar Doležalová, Ivana
http://linked.open...n/vavai/riv/zamer	Variabilita složek a interakcí v rostlinném patosystému a vliv faktorů prostředí na jejich projev
issn	1110-7251
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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