About: Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells—specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide-PEG-hydrazide, and subsequently anti-epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti-EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied in an Ephesia chip and an evaluation was performed by the MCF7 cell line. In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells—specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide-PEG-hydrazide, and subsequently anti-epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti-EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied in an Ephesia chip and an evaluation was performed by the MCF7 cell line. (en)
Title	Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture (en)
skos:prefLabel	Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture (en)
skos:notation	RIV/61389013:_____/14:00427443!RIV15-AV0-61389013
http://linked.open...avai/riv/aktivita	I P R
http://linked.open...avai/riv/aktivity	I, P(7E09109), R
http://linked.open...iv/cisloPeriodika	2-3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Horák, Daniel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	3712
http://linked.open...ai/riv/idVysledku	RIV/61389013:_____/14:00427443
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	biofunctionalization; circulating tumor cells; EpCAM (en)
http://linked.open.../riv/klicoveSlovo	circulating tumor cells EpCAM biofunctionalization
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[8807ACC40BB9]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CD
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated Micro-Nano-Opto Fluidic systems for high-content diagnosis and studies of rare cancer cells
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	35
http://linked.open...iv/tvurceVysledku	Horák, Daniel Svobodová, Z. Bílková, Z. Kučerová, J. Bruckova, L. Autebert, J. Viovy, J.-L.
http://linked.open...ain/vavai/riv/wos	000331899400012
issn	0173-0835
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/elps.201300196

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