About: Microarray analysis using limited amount of cells

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to more precisely analyze defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The HL60 cell line was used in the search for a suitable protocol that can be used for clinical samples of CD34+ hematopoietic cells obtained from bone marrow. The goal was to discover the best method of isolating and amplifying RNA from a small number of cells. Our evaluation of various methods and kits available in the market revealed that the combination of RNAqueous Kit (Ambion, USA) for RNA isolation and the SenseAmp Plus Kit (Genisphere, USA) for one-round RNA amplification produced the best results. cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to more precisely analyze defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The HL60 cell line was used in the search for a suitable protocol that can be used for clinical samples of CD34+ hematopoietic cells obtained from bone marrow. The goal was to discover the best method of isolating and amplifying RNA from a small number of cells. Our evaluation of various methods and kits available in the market revealed that the combination of RNAqueous Kit (Ambion, USA) for RNA isolation and the SenseAmp Plus Kit (Genisphere, USA) for one-round RNA amplification produced the best results. (en)
Title	Microarray analysis using limited amount of cells Microarray analysis using limited amount of cells (en)
skos:prefLabel	Microarray analysis using limited amount of cells Microarray analysis using limited amount of cells (en)
skos:notation	RIV/00216224:14330/09:00034548!RIV11-MSM-14330___
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(2B06052), P(LC535), V, Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	55
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Kozubek, Michal Potěšilová, Michaela Hrabčáková, Viera Doubek, Michael Mayer, Jiří Krontorád Koutná, Irena Klabusay, Martin Tesařová, Lenka Peterková, Martina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	326151
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/09:00034548
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cDNA microarray; RNA isolation; RNA amplifiacation; CD34+ cells (en)
http://linked.open.../riv/klicoveSlovo	CD34+ cells RNA isolation RNA amplifiacation cDNA microarray
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[1EB31851CE7A]
http://linked.open...i/riv/nazevZdroje	Folia Biologica (Praha)
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	9 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	2009
http://linked.open...iv/tvurceVysledku	Doubek, Michael Mayer, Jiří Kozubek, Michal Potěšilová, Michaela Klabusay, Martin Krontorád Koutná, Irena Tesařová, Lenka Peterková, Martina Hrabčáková, Viera
http://linked.open...ain/vavai/riv/wos	000265765100004
http://linked.open...n/vavai/riv/zamer	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	0015-5500
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14330

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