About: Rapid and effective method for the separation of Bacillus subtilis vegetative cells and spores

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About: Rapid and effective method for the separation of Bacillus subtilis vegetative cells and spores Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/article/10.1007%2Fs12223-012-0157-y
Description	The separation of vegetative cells from spores coexisting in Bacillus subtilis stationary phase cultures is a key step when studying both of the subpopulations. Lysozyme treatment was used for breaking down the vegetative cells. Unaffected spores were sedimented and separated by centrifugation. Characterization of the cell-free supernatant showed that it represents membrane and cytoplasmic fractions and, thus, can readily be used for determining their biochemical and microbiological properties. The separation efficacy, selectivity and suitability of the method were verified by observation in microscope, CFU counting, and by analysis of the phospholipid composition as well. The separation of vegetative cells from spores coexisting in Bacillus subtilis stationary phase cultures is a key step when studying both of the subpopulations. Lysozyme treatment was used for breaking down the vegetative cells. Unaffected spores were sedimented and separated by centrifugation. Characterization of the cell-free supernatant showed that it represents membrane and cytoplasmic fractions and, thus, can readily be used for determining their biochemical and microbiological properties. The separation efficacy, selectivity and suitability of the method were verified by observation in microscope, CFU counting, and by analysis of the phospholipid composition as well. (en)
Title	Rapid and effective method for the separation of Bacillus subtilis vegetative cells and spores Rapid and effective method for the separation of Bacillus subtilis vegetative cells and spores (en)
skos:prefLabel	Rapid and effective method for the separation of Bacillus subtilis vegetative cells and spores Rapid and effective method for the separation of Bacillus subtilis vegetative cells and spores (en)
skos:notation	RIV/00216208:11310/12:10127889!RIV13-MSM-11310___
http://linked.open...avai/riv/aktivita	I Z
http://linked.open...avai/riv/aktivity	I, Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Seydlová, Gabriela Svobodová, Jaroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	164086
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10127889
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	stationary phase; spore separation; phospholipids; lysozyme; Bacillus subtilis (en)
http://linked.open.../riv/klicoveSlovo	Bacillus subtilis stationary phase spore separation phospholipids lysozyme
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[DBA06ED95062]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Svobodová, Jaroslava Seydlová, Gabriela
http://linked.open...ain/vavai/riv/wos	000307509300010
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	0015-5632
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s12223-012-0157-y
http://localhost/t...ganizacniJednotka	11310

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