About: Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples

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About: Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1002/elan.201400449/abstract
Description	Electrochemical methods hold promise for fast and inexpensive determination of cancer biomarkers, including microRNAs playing an important role in regulation of gene expression. We developed a simple assay utilizing (i) microRNA labeling with osmium(VI) complexes, (ii) hybridization of microRNA with magnetic bead-immobilized capture probe, and (iii) adsorptive transfer stripping (AdTS) technique in which working electrode is dipped into a microliter-sized drop for direct adsorption of microRNA. Increased sensitivity and decreased sample consumption was achieved, and nanogram quantities of microRNA present in 500-fold excess of total RNA were determined. Our results indicate only negligible interference during hybridization from other RNA molecules. Electrochemical methods hold promise for fast and inexpensive determination of cancer biomarkers, including microRNAs playing an important role in regulation of gene expression. We developed a simple assay utilizing (i) microRNA labeling with osmium(VI) complexes, (ii) hybridization of microRNA with magnetic bead-immobilized capture probe, and (iii) adsorptive transfer stripping (AdTS) technique in which working electrode is dipped into a microliter-sized drop for direct adsorption of microRNA. Increased sensitivity and decreased sample consumption was achieved, and nanogram quantities of microRNA present in 500-fold excess of total RNA were determined. Our results indicate only negligible interference during hybridization from other RNA molecules. (en)
Title	Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples (en)
skos:prefLabel	Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples (en)
skos:notation	RIV/00209805:_____/14:#0000564!RIV15-GA0-00209805
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED2.1.00/03.0101), P(GA13-00956S), P(GP14-24931P)
http://linked.open...iv/cisloPeriodika	October 31
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hrstka, Roman Paleček, Emil Vojtěšek, Bořivoj Bartošík, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykův onkologický ústav
http://linked.open...dnocenehoVysledku	1713
http://linked.open...ai/riv/idVysledku	RIV/00209805:_____/14:#0000564
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cancer; mercury electrode; microRNA; voltammetry (en)
http://linked.open.../riv/klicoveSlovo	cancer mercury electrode voltammetry microRNA
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[BCA9D96A8630]
http://linked.open...i/riv/nazevZdroje	Electroanalysis
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Emerging roles for the proteins of Anterior Gradient family in cancer development Regional Centre for Applied Molecular Oncology (RECAMO) Electrochemical methods for bioanalysis of nucleic acids and their application in cancer diagnostics
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Hrstka, Roman Vojtěšek, Bořivoj Paleček, Emil Bartošík, Martin
http://linked.open...ain/vavai/riv/wos	000345978300002
issn	1040-0397
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/elan.201400449

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