About: Detection of genetic variability in sesame (Sesamum indicum L.) by a random amplified polymorphic DNA assay     Goto   Sponge   NotDistinct   Permalink

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Description
  • RAPD (náhodně amplifikované polymorfní DNA) markery byly použity k identifikaci 6 odrůd, patnácti F1 a patnácti F2 populací sezamu. Hybridní populace byly výsledkem polovičního dialelního křížení šesti odrůd. K amplifikaci DNA bylo použito celkem 8 náhodných primerů (dekamerů). Tyto primery neumožnily odlišit šest rodičovských genotypů. Profily F1 a F2 populací byly shodné s profily rodičů. Vyjímku tvořily dvě F2 populace, u kterých byl byl přítomen nový produkt RAPD dlouhý přibližně 850 bp. Tento výsledek by mohl být způsoben vysokou genetickou podobností vybraných odrůd sezamu vyvolanou předchozí selekcí anebo heterogenitou použitých odrůd (víceliniové). (cs)
  • Random amplified polymorphic DNA (RAPD) markers were used to identify six varieties, fifteen F1 and fifteen F2 populations of sesame. The hybrid populations were the result of an one-way diallel cross program among the six varieties. Eight arbitrary lO-mer primers were used for amplification of sesame DNA. These primers did not allow us to differentiate the six varieties of sesame. RAPD patterns of F1 populations were identical to profiles of their parents. This could be caused by high genetic similarity of accessions tested, previous selection and/or genetic heterogeneity of used varieties (more than one line). A specific fragment was found in two F2 populations.
  • Random amplified polymorphic DNA (RAPD) markers were used to identify six varieties, fifteen F1 and fifteen F2 populations of sesame. The hybrid populations were the result of an one-way diallel cross program among the six varieties. Eight arbitrary lO-mer primers were used for amplification of sesame DNA. These primers did not allow us to differentiate the six varieties of sesame. RAPD patterns of F1 populations were identical to profiles of their parents. This could be caused by high genetic similarity of accessions tested, previous selection and/or genetic heterogeneity of used varieties (more than one line). A specific fragment was found in two F2 populations. (en)
Title
  • Detection of genetic variability in sesame (Sesamum indicum L.) by a random amplified polymorphic DNA assay
  • Detection of genetic variability in sesame (Sesamum indicum L.) by a random amplified polymorphic DNA assay (en)
  • Detekce genetické variability sezamu (Sesamum indicum L.) metodou náhodně amplifikovaných polymorfních DNA (cs)
skos:prefLabel
  • Detection of genetic variability in sesame (Sesamum indicum L.) by a random amplified polymorphic DNA assay
  • Detection of genetic variability in sesame (Sesamum indicum L.) by a random amplified polymorphic DNA assay (en)
  • Detekce genetické variability sezamu (Sesamum indicum L.) metodou náhodně amplifikovaných polymorfních DNA (cs)
skos:notation
  • RIV/00027006:_____/03:860!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 593;596
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE-M01-01-03)
http://linked.open...iv/cisloPeriodika
  • 12
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 603268
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/03:860
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • sesame; genetic variability; RAPD markers; identification of genotypes (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [2E1C409BCC1F]
http://linked.open...i/riv/nazevZdroje
  • Agriculture
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 49
http://linked.open...iv/tvurceVysledku
  • Salava, Jaroslav
  • Veverka, Karel
  • Ammar, S.
  • El-Ashary, M.
  • El-Bramway, M.
  • El-Sattar, M.
  • El-Shazly, M.
http://linked.open...n/vavai/riv/zamer
issn
  • 0002-1695
number of pages
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