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  • Researchers have found they can control the size of densely packed DNA structures by changing the salt concentration in solutions containing DNA. The finding could improve the efficiency of gene delivery for medical treatment and disease prevention. In this paper the condensation of 10kbp plasmid DNA, induced by addition of NaCl, KCl and MgCl2 salts in a range of concentrations (0-150 mM) using time correlated single photon counting fluorescence correlation spectroscopy (TCSPC-FCS) was studied. The dependence of diffusion time (τD) on Csalt displays two regimes, a salt-independent at low C salt and salt-dependent at higher salt concentration. In the salt-dependent regime τD decreases sharply with increase of C salt. That means that due to the partial neutralization of the DNA charge by Na+, K+ or Mg2+ ions a lower dicephalic surfactants (DCPh) concentrations are needed to provoke condensation in the salt-independent regime compared to the related case of DNA condensation by the same dicephalic surfactant.
  • Researchers have found they can control the size of densely packed DNA structures by changing the salt concentration in solutions containing DNA. The finding could improve the efficiency of gene delivery for medical treatment and disease prevention. In this paper the condensation of 10kbp plasmid DNA, induced by addition of NaCl, KCl and MgCl2 salts in a range of concentrations (0-150 mM) using time correlated single photon counting fluorescence correlation spectroscopy (TCSPC-FCS) was studied. The dependence of diffusion time (τD) on Csalt displays two regimes, a salt-independent at low C salt and salt-dependent at higher salt concentration. In the salt-dependent regime τD decreases sharply with increase of C salt. That means that due to the partial neutralization of the DNA charge by Na+, K+ or Mg2+ ions a lower dicephalic surfactants (DCPh) concentrations are needed to provoke condensation in the salt-independent regime compared to the related case of DNA condensation by the same dicephalic surfactant. (en)
Title
  • The effects of salts on dicephalic based condensing agents.
  • The effects of salts on dicephalic based condensing agents. (en)
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  • The effects of salts on dicephalic based condensing agents.
  • The effects of salts on dicephalic based condensing agents. (en)
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  • RIV/68407700:21460/12:00202772!RIV13-MSM-21460___
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  • P(EE.2.3.20.0092)
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  • 133489
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  • RIV/68407700:21460/12:00202772
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  • DNA structures; bacteria; viruses; salt; fluorescence (en)
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  • [225C2993A369]
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  • Kladno
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  • Prague
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  • Instuments and Methods for Biology and Medicine 2012
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  • Macháň, Radek
  • Kral, T.
  • Williamson, Philip
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number of pages
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  • České vysoké učení technické v Praze
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  • 978-80-01-05119-1
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  • 21460
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