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Description
| - Lidské osteoblasty (SAOS-2) byly kultivovány na jednostěnných uhlíkových nanotubách (SWCNT) na nanokrystalickém diamantu (NCD) pro studium potenciálního využití těchto materiálů pro biologické aplikace. V této práci jsou presentovány závislosti metabolické aktivity a morfologie osteoblastů na i) použitém materiálu (NCD nebo SWCNT filmy), ii) zakončení povrchu O-zakončení, které vede k hydrofilnímu povrchu a H-zakončení, které vede k hydrofobnímu povrchu, iii) hrubosti povrchu v rozsahu 11-39nm RMS a iv) způsobu přípravy SWCNT –laserová ablace, výboj a HiPco proces. U SWCNT filmů poklesla metabolická aktivita na netoxickou úroveň stejně jako u H-zakončených NCD filmů a u polystyrenu určeného k pěstování buněk. Metabolická aktivita rozte v případě O-zakončených NCD filmů v závislosti na jejich drsnosti. Morfologická studie potvrdila lepší vlastnosti O-zakončených NCD filmů. (cs)
- Human osteoblasts (SAOS-2) are cultured on single-walled carbon nanotube (SWCNT) and nanocrystalline diamond (NCD) films in order to evaluate their potential for biological application. We present dependence of metabolic activity and morphology of osteoblasts on (i) material substrate (NCD or SWCNT films), (ii) surface termination – O-termination causing hydrophilic surface and H-termination (as deposited NCDs) causing hydrophobic surface, (iii) surface roughness in the range of 11–39 nm RMS and (iv) SWCNT production technique – laser ablation, arc-discharge evaporation and HiPco. The metabolic activity decreases to the same non-toxic level on SWCNT films produced by different techniques as well as on H-terminated NCD compared to tissue culture polystyrene. The metabolic activity is increasing on O-terminated NCD films as a function of their roughness. Morphological observations support superior properties of O-terminated NCD films.
- Human osteoblasts (SAOS-2) are cultured on single-walled carbon nanotube (SWCNT) and nanocrystalline diamond (NCD) films in order to evaluate their potential for biological application. We present dependence of metabolic activity and morphology of osteoblasts on (i) material substrate (NCD or SWCNT films), (ii) surface termination – O-termination causing hydrophilic surface and H-termination (as deposited NCDs) causing hydrophobic surface, (iii) surface roughness in the range of 11–39 nm RMS and (iv) SWCNT production technique – laser ablation, arc-discharge evaporation and HiPco. The metabolic activity decreases to the same non-toxic level on SWCNT films produced by different techniques as well as on H-terminated NCD compared to tissue culture polystyrene. The metabolic activity is increasing on O-terminated NCD films as a function of their roughness. Morphological observations support superior properties of O-terminated NCD films. (en)
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Title
| - The effect of SWCNT and nano-diamond films on human osteoblast cells
- The effect of SWCNT and nano-diamond films on human osteoblast cells (en)
- Vliv SWCNT a nano-diamantu na lidské osteoblasty (cs)
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skos:prefLabel
| - The effect of SWCNT and nano-diamond films on human osteoblast cells
- The effect of SWCNT and nano-diamond films on human osteoblast cells (en)
- Vliv SWCNT a nano-diamantu na lidské osteoblasty (cs)
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skos:notation
| - RIV/68378271:_____/07:00090792!RIV08-AV0-68378271
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(KAN400100701), P(KJB400400601), Z(AV0Z10100521), Z(AV0Z40400503)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68378271:_____/07:00090792
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - SWCNT; nano-diamond films; human osteoblast cells (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Physica Status Solidi B-Basic Solid State Physics
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Dunsch, L.
- Kalbáč, Martin
- Kalbáčová, M.
- Kromka, Alexander
- Rezek, Bohuslav
- Kmoch, S.
- Vaněček, Milan
- Hempel, U.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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