About: Raman tweezers in microfluidic systems for analysis and sorting of living cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment, dedicated to identification and sorting of biological objects, such as living cells of various unicellular organisms. Our main goal was to create a robust and universal platform for non-destructive and non-contact sorting of micro-objects based on their Raman spectral properties. This approach allowed us to collect spectra containing information about the chemical composition of the objects, such as the presence and composition of pigments, lipids, proteins, or nucleic acids, avoiding artificial chemical probes such as fluorescent markers. The non-destructive nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used a mixture of polystyrene micro-particles and algal cells to test and demonstrate the function of our analytical and sorting system. The devised system could find its use in many medical, biotechnological, and biological applications. We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment, dedicated to identification and sorting of biological objects, such as living cells of various unicellular organisms. Our main goal was to create a robust and universal platform for non-destructive and non-contact sorting of micro-objects based on their Raman spectral properties. This approach allowed us to collect spectra containing information about the chemical composition of the objects, such as the presence and composition of pigments, lipids, proteins, or nucleic acids, avoiding artificial chemical probes such as fluorescent markers. The non-destructive nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used a mixture of polystyrene micro-particles and algal cells to test and demonstrate the function of our analytical and sorting system. The devised system could find its use in many medical, biotechnological, and biological applications. (en)
Title	Raman tweezers in microfluidic systems for analysis and sorting of living cells Raman tweezers in microfluidic systems for analysis and sorting of living cells (en)
skos:prefLabel	Raman tweezers in microfluidic systems for analysis and sorting of living cells Raman tweezers in microfluidic systems for analysis and sorting of living cells (en)
skos:notation	RIV/68081731:_____/14:00437742!RIV15-TA0-68081731
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED0017/01/01), P(FR-TI1/433), P(LO1212), P(TA03010642)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Ježek, Jan Zemánek, Pavel Pilát, Zdeněk Kaňka, Jan
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav přístrojové techniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	41275
http://linked.open...ai/riv/idVysledku	RIV/68081731:_____/14:00437742
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cells; chemicals; fluorescent markers; optical analysis; optical trappingo; organisms; Raman spectroscopy; proteins (en)
http://linked.open.../riv/klicoveSlovo	chemicals organisms Raman spectroscopy cells proteins optical analysis fluorescent markers optical trappingo
http://linked.open...ontrolniKodProRIV	[A624BEB246A5]
http://linked.open...v/mistoKonaniAkce	Jelenia Góra
http://linked.open...i/riv/mistoVydani	Bellingham
http://linked.open...i/riv/nazevZdroje	19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE 9441)
http://linked.open...in/vavai/riv/obor	BH
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	ALISI - Centre of advanced diagnostic methods and technologies APPLICATION LABORATORIES OF ADVANCED MICROTECHNOLOGIES AND NANOTECHNOLOGIES *Development of instrumentation and methodology for the selection of photoautotrophic microorganisms for production of higher-generation biofuels Advanced microfluidic techniques
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Ježek, Jan Zemánek, Pavel Kaňka, Jan Pilát, Zdeněk
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2014-09-08 (xsd:date)
issn	0277-786X
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1117/12.2176047
http://purl.org/ne...btex#hasPublisher	SPIE
https://schema.org/isbn	9781628415568

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