About: Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release     Goto   Sponge   Distinct   Permalink

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Description
  • We demonstrate a simple and rapid method for the oriented immobilization of peptide-N4-(Nacetyl- glucosaminyl) asparagine amidase F (PNGase F) on a porous polymer monolith. The oriented immobilization is based on the affinity of glutathione-S-transferase (GST) tagged PNGase F towards glutathione modified monolith prepared in the capillary format. This approach allows the oriented and easily replaceable immobilization of PNGase F for rapid and efficient release of N-linked glycans. The reactor was tested by deglycosylation of several native glycoproteins such as ribonuclease B, fetuin or human immunoglobulin G. The proteins were effectively deglycosylated in several minutes and the released N-linked glycans were analyzed using off-line MALDI/MS or CE/LIF.
  • We demonstrate a simple and rapid method for the oriented immobilization of peptide-N4-(Nacetyl- glucosaminyl) asparagine amidase F (PNGase F) on a porous polymer monolith. The oriented immobilization is based on the affinity of glutathione-S-transferase (GST) tagged PNGase F towards glutathione modified monolith prepared in the capillary format. This approach allows the oriented and easily replaceable immobilization of PNGase F for rapid and efficient release of N-linked glycans. The reactor was tested by deglycosylation of several native glycoproteins such as ribonuclease B, fetuin or human immunoglobulin G. The proteins were effectively deglycosylated in several minutes and the released N-linked glycans were analyzed using off-line MALDI/MS or CE/LIF. (en)
Title
  • Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release
  • Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release (en)
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  • Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release
  • Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release (en)
skos:notation
  • RIV/68081715:_____/12:00386014!RIV13-AV0-68081715
http://linked.open...avai/predkladatel
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  • I
http://linked.open...vai/riv/dodaniDat
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  • 157114
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  • RIV/68081715:_____/12:00386014
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  • PNGase F; oriented immobilization; monolith (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [D879290E2330]
http://linked.open...v/mistoKonaniAkce
  • Brno
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  • Brno
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  • CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis
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  • Foret, František
  • Křenková, Jana
  • Guttman, A.
  • Keresztessy, Z.
  • Szekrényes, A.
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
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  • Ústav analytické chemie AV ČR, v. v. i
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  • 978-80-904959-1-3
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