About: Magnetic bead-based hybridization assay for electrochemical detection of microRNA

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Aberrant expression of microRNAs (miRNAs), short non-coding RNA molecules regulating gene expression, is often found in tumor cells, making the miRNAs suitable candidates as cancer biomarkers. Electrochemistry is an interesting alternative to current standard methods of miRNA detection by offering cheaper instrumentation and faster assays times. In this paper, we labeled miRNA in a quick, simple, two-step procedure with electroactive complex of osmium(VI) and 2,2'-bipyridine, Os(VI) bipy, which specifically binds to the ribose at the 3'-end of the miRNA, and hybridized such labeled miRNA with biotinylated capture probe attached to the streptavidin magnetic beads. Labeled miRNA was then detected at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of the peak from the Os(VI) bipy label. Aberrant expression of microRNAs (miRNAs), short non-coding RNA molecules regulating gene expression, is often found in tumor cells, making the miRNAs suitable candidates as cancer biomarkers. Electrochemistry is an interesting alternative to current standard methods of miRNA detection by offering cheaper instrumentation and faster assays times. In this paper, we labeled miRNA in a quick, simple, two-step procedure with electroactive complex of osmium(VI) and 2,2'-bipyridine, Os(VI) bipy, which specifically binds to the ribose at the 3'-end of the miRNA, and hybridized such labeled miRNA with biotinylated capture probe attached to the streptavidin magnetic beads. Labeled miRNA was then detected at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of the peak from the Os(VI) bipy label. (en)
Title	Magnetic bead-based hybridization assay for electrochemical detection of microRNA Magnetic bead-based hybridization assay for electrochemical detection of microRNA (en)
skos:prefLabel	Magnetic bead-based hybridization assay for electrochemical detection of microRNA Magnetic bead-based hybridization assay for electrochemical detection of microRNA (en)
skos:notation	RIV/68081707:_____/14:00427971!RIV15-GA0-68081707
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA13-00956S), P(GBP206/12/G151)
http://linked.open...iv/cisloPeriodika	FEB2014
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Paleček, Emil Bartošík, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	26878
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/14:00427971
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MicroRNA; Electrochemistry; Mercury electrodes (en)
http://linked.open.../riv/klicoveSlovo	Electrochemistry MicroRNA Mercury electrodes
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[50F0694E7D80]
http://linked.open...i/riv/nazevZdroje	Analytica Chimica Acta
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of novel approaches to bioanalysis and molecular diagnostics Emerging roles for the proteins of Anterior Gradient family in cancer development
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	813
http://linked.open...iv/tvurceVysledku	Paleček, Emil Bartošík, Martin Hrstka, R. Vojtešek, B.
http://linked.open...ain/vavai/riv/wos	000331556000004
issn	0003-2670
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.aca.2014.01.023

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