About: A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous     Goto   Sponge   Distinct   Permalink

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  • Nicotiana tabacum (tobacco, 2n?=?4x?=?48) is an allotetraploid with 24 S-genome chromosomes (from a diploid related to N.?sylvestris) and 24 T-genome chromosomes (from a diploid related to N.?tomentosiformis). The BY-2 suspension cell culture, derived from N.?tabacum cultivar Bright Yellow 2, has been used extensively for research in molecular and cell biology for almost 40 years; a Web of Knowledge search reveals that it has been used over 150 times since 2008 alone, largely for cell cycle and plant physiology studies. However, we show that this culture is unstable and, as with other long-term cultures, exists as a community of cells with different karyotypes reflected in different chromosome numbers, morphologies and distributions of satellite repeats.
  • Nicotiana tabacum (tobacco, 2n?=?4x?=?48) is an allotetraploid with 24 S-genome chromosomes (from a diploid related to N.?sylvestris) and 24 T-genome chromosomes (from a diploid related to N.?tomentosiformis). The BY-2 suspension cell culture, derived from N.?tabacum cultivar Bright Yellow 2, has been used extensively for research in molecular and cell biology for almost 40 years; a Web of Knowledge search reveals that it has been used over 150 times since 2008 alone, largely for cell cycle and plant physiology studies. However, we show that this culture is unstable and, as with other long-term cultures, exists as a community of cells with different karyotypes reflected in different chromosome numbers, morphologies and distributions of satellite repeats. (en)
Title
  • A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous
  • A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous (en)
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  • A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous
  • A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous (en)
skos:notation
  • RIV/68081707:_____/12:00389788!RIV13-AV0-68081707
http://linked.open...avai/predkladatel
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  • I, P(GAP501/10/0208)
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  • 3
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  • 120512
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  • RIV/68081707:_____/12:00389788
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  • cell biology; cell cycle; evolution (en)
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  • US - Spojené státy americké
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  • [8DA0CCE71089]
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  • Botanical Journal of the Linnean Society
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  • 170
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  • Kovařík, Aleš
  • Leitch, A. R.
  • Matyášek, Roman
  • Lim, K. Y.
  • Součková, Kamila
http://linked.open...ain/vavai/riv/wos
  • 000310079500007
issn
  • 0024-4074
number of pages
http://bibframe.org/vocab/doi
  • 10.1111/j.1095-8339.2012.01280.x
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