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Description
| - Místně specifická chemická modifikace je užitečná technologie při charakterizaci bílkovin. Počet dostupných chemických sond pro strukturu bílkovin, které reagují s bílkovinami za mírných podmínek ve vodných roztocích, je značně omezený. V této práci jsme studovali reakci komplexu oxid osmičelý, 2,2´-bipyridin (Os,bipy) s několika peptidy za využití kapilární zonální elektroforézy (CZE) a hmotové spektrometrie MALDI-TOF (matrix-assisted laser desorption-ionisation–time-of-flight). Oběma technikami byla prokázána tvorba stabilního komplexu Os,bipy s tryptofanovými zbytky. Při CZE byly pozorovány píky s rozdílnými dobami putování a rozdílnými spektry UV a viditelné oblasti. MALDI-TOF hmotová spektrometrie prokázala tvorbu produktu s charakteristickým isotopovým obrazem, který odpovídá přítomnosti osmiového atomu. (cs)
- Site-specific chemical modification is a useful technology in characterisation of proteins, but the number of chemical probes of the protein structure reacting with proteins under mild conditions in aqueous solutions is rather limited. Here we studied the reaction of osmium tetroxide, 2,2'-bipyridine (Os,bipy) with several peptides using capillary zone electrophoresis (CZE) and matrix-assisted laser desorption-ionisation-time-of-flight mass spectrometry (MALDI-TOF MS). Both techniques showed formation of a stable complex of Os,bipy with tryptophan residues. In CZE peaks with different migration times and UV-Vis spectra were observed. MALDI-TOF MS showed the formation of a product with characteristic isotopic pattern corresponding to the presence of osmium atom.
- Site-specific chemical modification is a useful technology in characterisation of proteins, but the number of chemical probes of the protein structure reacting with proteins under mild conditions in aqueous solutions is rather limited. Here we studied the reaction of osmium tetroxide, 2,2'-bipyridine (Os,bipy) with several peptides using capillary zone electrophoresis (CZE) and matrix-assisted laser desorption-ionisation-time-of-flight mass spectrometry (MALDI-TOF MS). Both techniques showed formation of a stable complex of Os,bipy with tryptophan residues. In CZE peaks with different migration times and UV-Vis spectra were observed. MALDI-TOF MS showed the formation of a product with characteristic isotopic pattern corresponding to the presence of osmium atom. (en)
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Title
| - Derivatisation of peptides with osmium tetroxide, 2,2´-bipyridine: capillary electrophoretic and MALDI-TOF mass spectrometric study
- Derivatizace peptidů komplexem oxid osmičelý, 2,2´-bipyridin: studie kapilární elektroforézou a MALDI-TOF hmotovou spektrometrií (cs)
- Derivatisation of peptides with osmium tetroxide, 2,2´-bipyridine: capillary electrophoretic and MALDI-TOF mass spectrometric study (en)
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skos:prefLabel
| - Derivatisation of peptides with osmium tetroxide, 2,2´-bipyridine: capillary electrophoretic and MALDI-TOF mass spectrometric study
- Derivatizace peptidů komplexem oxid osmičelý, 2,2´-bipyridin: studie kapilární elektroforézou a MALDI-TOF hmotovou spektrometrií (cs)
- Derivatisation of peptides with osmium tetroxide, 2,2´-bipyridine: capillary electrophoretic and MALDI-TOF mass spectrometric study (en)
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skos:notation
| - RIV/68081707:_____/04:00104696!RIV/2005/AV0/A17005/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA204/03/0566), P(GA305/03/1100), Z(AV0Z5004920)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68081707:_____/04:00104696
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - peptide derivatisation; osmium tetroxide; CZE (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Paleček, Emil
- Billová, Sabina
- Havel, J.
- Šedo, O.
- Peńa-Méndez, E. M.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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