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Description
  • A set of tools for the genetic manipulation of the osmotolerant yeast Zygosaccharomyces rouxii was developed. Auxotrophic mutants (ura3 leu2, ura3 ade2, ura3 leu2 ade2) derived from the CBS 732 type strain were prepared. Centromeric and episomal Z. rouxii/E. coli shuttle plasmids with different marker genes (ScURA3, ZrLEU2, ZrADE2) and with multiple cloning sites were constructed, together with a plasmid enabling GFP-tagging. A system for repeatable targeted gene deletion in Z. rouxii was established
  • A set of tools for the genetic manipulation of the osmotolerant yeast Zygosaccharomyces rouxii was developed. Auxotrophic mutants (ura3 leu2, ura3 ade2, ura3 leu2 ade2) derived from the CBS 732 type strain were prepared. Centromeric and episomal Z. rouxii/E. coli shuttle plasmids with different marker genes (ScURA3, ZrLEU2, ZrADE2) and with multiple cloning sites were constructed, together with a plasmid enabling GFP-tagging. A system for repeatable targeted gene deletion in Z. rouxii was established (en)
  • Byl připraven soubor nástrojů genového inženýrství pro kvasinku Zygosaccharomyces rouxii. Byly zkonstruovány auxotrofní mutantní kmeny spolu s centromerovými a episomálními vektory a vyvinut system umožňující opakovatelné delece (cs)
Title
  • Tools for the genetic manipulation of Zygosaccharomyces rouxii
  • Nástroje genového inženýrství pro kvasinku Zygosaccharomyces rouxii (cs)
  • Tools for the genetic manipulation of Zygosaccharomyces rouxii (en)
skos:prefLabel
  • Tools for the genetic manipulation of Zygosaccharomyces rouxii
  • Nástroje genového inženýrství pro kvasinku Zygosaccharomyces rouxii (cs)
  • Tools for the genetic manipulation of Zygosaccharomyces rouxii (en)
skos:notation
  • RIV/67985823:_____/07:00091032!RIV08-AV0-67985823
http://linked.open.../vavai/riv/strany
  • 1285;1294
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA204/05/0028), Z(AV0Z50110509)
http://linked.open...iv/cisloPeriodika
  • 8
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 455322
http://linked.open...ai/riv/idVysledku
  • RIV/67985823:_____/07:00091032
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Zygosaccharomyces rouxii; genetic tools; plasmids (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [C2420E81FCCB]
http://linked.open...i/riv/nazevZdroje
  • FEMS Yeast Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 7
http://linked.open...iv/tvurceVysledku
  • Přibylová, Lenka
  • Sychrová, Hana
  • de Montigny, J.
http://linked.open...n/vavai/riv/zamer
issn
  • 1567-1356
number of pages
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