About: Tyramine content and enterococci isolated from the Slovak ewe's cheeses as a potential source of biogenic amines     Goto   Sponge   Distinct   Permalink

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  • In this study, the presence of Enterococcus spp. grown on Slanetz-Bartley agar plates from cheeses made from ewe's milk was investigated. The Gram positive catalase negative bacilli identified in this study belonged to the Enterococcus species. A multiplex PCR reaction was used for confirmation of isolated bacteria. Out of 34 isolates, 17 strains (50%) were identified as Enterococcus spp. In further investigation the confirmed isolated strains by PCR method (88,24% E. faecalis and 5,88% E. faecium, and 5,88% Enterococcus spp.) were studied for the presence of tyrdc gene responsible for tyramine production. In all isolated strains of Enetococcus spp. (100%) were found to carry the tyrdc gene. The same results were achieved with the tyramine production by the isolated strains in medium according to Maijala after incubation at l5°C and 6°C for l,2,3, and 6 days. The detected content of tyramine in the ewe'milk lump cheeses included in the experiment reached the mean value of 24,27 mg kg-1 (3,70 - 68,20 mg kg-1).
  • In this study, the presence of Enterococcus spp. grown on Slanetz-Bartley agar plates from cheeses made from ewe's milk was investigated. The Gram positive catalase negative bacilli identified in this study belonged to the Enterococcus species. A multiplex PCR reaction was used for confirmation of isolated bacteria. Out of 34 isolates, 17 strains (50%) were identified as Enterococcus spp. In further investigation the confirmed isolated strains by PCR method (88,24% E. faecalis and 5,88% E. faecium, and 5,88% Enterococcus spp.) were studied for the presence of tyrdc gene responsible for tyramine production. In all isolated strains of Enetococcus spp. (100%) were found to carry the tyrdc gene. The same results were achieved with the tyramine production by the isolated strains in medium according to Maijala after incubation at l5°C and 6°C for l,2,3, and 6 days. The detected content of tyramine in the ewe'milk lump cheeses included in the experiment reached the mean value of 24,27 mg kg-1 (3,70 - 68,20 mg kg-1). (en)
Title
  • Tyramine content and enterococci isolated from the Slovak ewe's cheeses as a potential source of biogenic amines
  • Tyramine content and enterococci isolated from the Slovak ewe's cheeses as a potential source of biogenic amines (en)
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  • Tyramine content and enterococci isolated from the Slovak ewe's cheeses as a potential source of biogenic amines
  • Tyramine content and enterococci isolated from the Slovak ewe's cheeses as a potential source of biogenic amines (en)
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  • RIV/62157124:16270/11:43870579!RIV12-MSM-16270___
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  • RIV/62157124:16270/11:43870579
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  • tyramine; tyrdc gene; cheeses; Enterococcus spp. (en)
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  • CZ - Česká republika
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  • Chemické listy - Special Issue
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  • 105
http://linked.open...iv/tvurceVysledku
  • Vorlová, Lenka
  • Karpíšková, Renata
  • Dičáková, Zuzana
  • Dudriková, Eva
  • Dušková, Marta
  • Gallo, Juraj
  • Pol'áková, Lucia
  • Trivedi, Krina
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  • 0009-2770
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