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  • The aim of this study was verification of the presence or absence of specific DNA sequences coding for tyrosine decarboxylase in starter cultures using for fermented sausages production and confirmation of the tyrosine decarboxylase gene expression using rapid HPLC analysis of the expression product - tyramin. Genomic DNA from single microorganisms using as starter cultures for fermented sausages was extracted using fenol extraction procedure. This DNA was used as a template for PCR identification. The set of oligonucleotide primers based on tyrosine decarboxylase gene sequence was used in PCR. Two strains with high tyrosine decarboxylase activity were detected (Lactobacillus curvatus and Lactobacillus sake). The tyrosine decarboxylase gene expression of these two strains was analyzed using optimalized rapid HPLC method which confirmed the presence of high concentrations of tyramine. These results show suitability of described PCR and HPLC method for the skríning of starter cultures for the presence
  • The aim of this study was verification of the presence or absence of specific DNA sequences coding for tyrosine decarboxylase in starter cultures using for fermented sausages production and confirmation of the tyrosine decarboxylase gene expression using rapid HPLC analysis of the expression product - tyramin. Genomic DNA from single microorganisms using as starter cultures for fermented sausages was extracted using fenol extraction procedure. This DNA was used as a template for PCR identification. The set of oligonucleotide primers based on tyrosine decarboxylase gene sequence was used in PCR. Two strains with high tyrosine decarboxylase activity were detected (Lactobacillus curvatus and Lactobacillus sake). The tyrosine decarboxylase gene expression of these two strains was analyzed using optimalized rapid HPLC method which confirmed the presence of high concentrations of tyramine. These results show suitability of described PCR and HPLC method for the skríning of starter cultures for the presence (en)
  • The aim of this study was verification of the presence or absence of specific DNA sequences coding for tyrosine decarboxylase in starter cultures using for fermented sausages production and confirmation of the tyrosine decarboxylase gene expression using rapid HPLC analysis of the expression product - tyramin. Genomic DNA from single microorganisms using as starter cultures for fermented sausages was extracted using fenol extraction procedure. This DNA was used as a template for PCR identification. The set of oligonucleotide primers based on tyrosine decarboxylase gene sequence was used in PCR. Two strains with high tyrosine decarboxylase activity were detected (Lactobacillus curvatus and Lactobacillus sake). The tyrosine decarboxylase gene expression of these two strains was analyzed using optimalized rapid HPLC method which confirmed the presence of high concentrations of tyramine. These results show suitability of described PCR and HPLC method for the skríning of starter cultures for the presence (cs)
Title
  • Využití HPLC ke stanovení produktu exprese genu pro mikrobiální tyrosindekarboxylasu
  • Využití HPLC ke stanovení produktu exprese genu pro mikrobiální tyrosindekarboxylasu (cs)
  • The use of HPLC for determination of expression product of microbial tyrosine decarboxylase (en)
skos:prefLabel
  • Využití HPLC ke stanovení produktu exprese genu pro mikrobiální tyrosindekarboxylasu
  • Využití HPLC ke stanovení produktu exprese genu pro mikrobiální tyrosindekarboxylasu (cs)
  • The use of HPLC for determination of expression product of microbial tyrosine decarboxylase (en)
skos:notation
  • RIV/62156489:43210/07:00114129!RIV08-MSM-43210___
http://linked.open.../vavai/riv/strany
  • 907;910
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 432100001)
http://linked.open...iv/cisloPeriodika
  • 11
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 460095
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/07:00114129
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • tyrosine decarboxylase; biogenic amines; tyramine (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [E440E8A411A3]
http://linked.open...i/riv/nazevZdroje
  • Chemické listy
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 101
http://linked.open...iv/tvurceVysledku
  • Dohnal, Vlastimil
  • Burdychová, Radka
http://linked.open...n/vavai/riv/zamer
issn
  • 0009-2770
number of pages
http://localhost/t...ganizacniJednotka
  • 43210
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