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Description
  • V této práci jsme optimalizovali experimentální podmínky pro separaci a stanovení rostlinných hormonů a jejich metabolitů pomocí kapalinové chromatografie spojené s UV-VIS/ hmotnostní detekcí. Separovaná směs byla složená z trans, cis zeatinu, trans zeatin ribosidu., glukosidu, aromatických cytokininů a jejich ribosidů, přírodního a syntetického auxinu a syntetických cytokininů jako je benzyladenin a kinetin. Optimalizované podmínky byly: složení mobilní fáze, průtok, teplota kolonového prostoru a gradient. Byly stanoveny detekční limity pro jednotlivé látky.Optimalizovaná technika byla následně užita k analýze rostlinného vzorku. (cs)
  • We aimed on optimizing experimental conditions to separate and determine not only plant hormones but also their metabolites by liquid chromatography coupled with UV-VIS/mass detector. The mixture we analyzed was composed from trans-zeatin, cis-zeatin, zeatin riboside ane glucoside, aromatic cytokinins and their ribosides, natural and synthetic auxins as indolyl acetic acid or naphtylacetic acid, and synthetic cytokinins as benzyladenine or kinetin. We have optimized composition of mobile phase and its flow rate, column temperature, and gradient elution to reach low detection limits. The limits were estimated as units and/or tens nM for the target molecules. The optimized technique was subsequently utilized to analysis of plant samples.
  • We aimed on optimizing experimental conditions to separate and determine not only plant hormones but also their metabolites by liquid chromatography coupled with UV-VIS/mass detector. The mixture we analyzed was composed from trans-zeatin, cis-zeatin, zeatin riboside ane glucoside, aromatic cytokinins and their ribosides, natural and synthetic auxins as indolyl acetic acid or naphtylacetic acid, and synthetic cytokinins as benzyladenine or kinetin. We have optimized composition of mobile phase and its flow rate, column temperature, and gradient elution to reach low detection limits. The limits were estimated as units and/or tens nM for the target molecules. The optimized technique was subsequently utilized to analysis of plant samples. (en)
Title
  • An analysis of plant hormones by liquid chromatography with UV/MS detection
  • Anylýza rostlinných hormonů pomocí kapalinové chromatografie s UV/MS detekcí (cs)
  • An analysis of plant hormones by liquid chromatography with UV/MS detection (en)
skos:prefLabel
  • An analysis of plant hormones by liquid chromatography with UV/MS detection
  • Anylýza rostlinných hormonů pomocí kapalinové chromatografie s UV/MS detekcí (cs)
  • An analysis of plant hormones by liquid chromatography with UV/MS detection (en)
skos:notation
  • RIV/62156489:43210/07:00110028!RIV08-GA0-43210___
http://linked.open.../vavai/riv/strany
  • 38;38
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1M06030), P(GA522/07/0692)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 409274
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/07:00110028
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • plant hormones; HPLC (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [6ACD89FBA629]
http://linked.open...v/mistoKonaniAkce
  • Vysoké Tatry, Slovenská republika
http://linked.open...i/riv/nazevZdroje
  • Recent advanced in plant biotechnology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Havel, Ladislav
  • Kizek, René
  • Šupálková, Veronika
  • Diopan, Václav
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Neuveden
https://schema.org/isbn
  • 978-80-89088-51-5
http://localhost/t...ganizacniJednotka
  • 43210
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