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Description
| - V této práci jsme optimalizovali experimentální podmínky pro separaci a stanovení rostlinných hormonů a jejich metabolitů pomocí kapalinové chromatografie spojené s UV-VIS/ hmotnostní detekcí. Separovaná směs byla složená z trans, cis zeatinu, trans zeatin ribosidu., glukosidu, aromatických cytokininů a jejich ribosidů, přírodního a syntetického auxinu a syntetických cytokininů jako je benzyladenin a kinetin. Optimalizované podmínky byly: složení mobilní fáze, průtok, teplota kolonového prostoru a gradient. Byly stanoveny detekční limity pro jednotlivé látky.Optimalizovaná technika byla následně užita k analýze rostlinného vzorku. (cs)
- We aimed on optimizing experimental conditions to separate and determine not only plant hormones but also their metabolites by liquid chromatography coupled with UV-VIS/mass detector. The mixture we analyzed was composed from trans-zeatin, cis-zeatin, zeatin riboside ane glucoside, aromatic cytokinins and their ribosides, natural and synthetic auxins as indolyl acetic acid or naphtylacetic acid, and synthetic cytokinins as benzyladenine or kinetin. We have optimized composition of mobile phase and its flow rate, column temperature, and gradient elution to reach low detection limits. The limits were estimated as units and/or tens nM for the target molecules. The optimized technique was subsequently utilized to analysis of plant samples.
- We aimed on optimizing experimental conditions to separate and determine not only plant hormones but also their metabolites by liquid chromatography coupled with UV-VIS/mass detector. The mixture we analyzed was composed from trans-zeatin, cis-zeatin, zeatin riboside ane glucoside, aromatic cytokinins and their ribosides, natural and synthetic auxins as indolyl acetic acid or naphtylacetic acid, and synthetic cytokinins as benzyladenine or kinetin. We have optimized composition of mobile phase and its flow rate, column temperature, and gradient elution to reach low detection limits. The limits were estimated as units and/or tens nM for the target molecules. The optimized technique was subsequently utilized to analysis of plant samples. (en)
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Title
| - An analysis of plant hormones by liquid chromatography with UV/MS detection
- Anylýza rostlinných hormonů pomocí kapalinové chromatografie s UV/MS detekcí (cs)
- An analysis of plant hormones by liquid chromatography with UV/MS detection (en)
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skos:prefLabel
| - An analysis of plant hormones by liquid chromatography with UV/MS detection
- Anylýza rostlinných hormonů pomocí kapalinové chromatografie s UV/MS detekcí (cs)
- An analysis of plant hormones by liquid chromatography with UV/MS detection (en)
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skos:notation
| - RIV/62156489:43210/07:00110028!RIV08-GA0-43210___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(1M06030), P(GA522/07/0692)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/07:00110028
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - plant hormones; HPLC (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
| - Vysoké Tatry, Slovenská republika
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http://linked.open...i/riv/nazevZdroje
| - Recent advanced in plant biotechnology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Adam, Vojtěch
- Havel, Ladislav
- Kizek, René
- Šupálková, Veronika
- Diopan, Václav
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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number of pages
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http://purl.org/ne...btex#hasPublisher
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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