About: Gene organization and molecular modeling of copper amine oxidase from Aspergillus niger

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Amine oxidase AO-I from Aspergillus niger AKU 3302 was reported to contain topa quinone as a cofactor, however, the study on p-nitrophe-nylhydrazine derivatized enzyme and purified active site peptides showed the presence of a carboxylate ester linkage of TPQ to a gluta-mate. The catalytic functionality of such a cross-linked cofactor has been shown unlikely by spectroscopic and voltammetric studies on synthe-sized model compounds. We have obtained resonance Raman spectra of native and substrate reduced AO-I showing that the catalytically active cofactor is unmodified TPQ. The primary structure of the enzyme (Gen-Bank U31869) has been reviewed and updated by repeated isolation and sequencing of AO-I cDNA. This allowed rectification of severalerrors that account for previously reported low homology to other amine oxi-dases in the regions around copper binding histididyl residues. The results were confirmed by cloning the ao-1 structural gene (GenBank AF362473). Analysis of the gene 5 -up Amine oxidase AO-I from Aspergillus niger AKU 3302 was reported to contain topa quinone as a cofactor, however, the study on p-nitrophe-nylhydrazine derivatized enzyme and purified active site peptides showed the presence of a carboxylate ester linkage of TPQ to a gluta-mate. The catalytic functionality of such a cross-linked cofactor has been shown unlikely by spectroscopic and voltammetric studies on synthe-sized model compounds. We have obtained resonance Raman spectra of native and substrate reduced AO-I showing that the catalytically active cofactor is unmodified TPQ. The primary structure of the enzyme (Gen-Bank U31869) has been reviewed and updated by repeated isolation and sequencing of AO-I cDNA. This allowed rectification of severalerrors that account for previously reported low homology to other amine oxi-dases in the regions around copper binding histididyl residues. The results were confirmed by cloning the ao-1 structural gene (GenBank AF362473). Analysis of the gene 5 -up (en)
Title	Gene organization and molecular modeling of copper amine oxidase from Aspergillus niger Gene organization and molecular modeling of copper amine oxidase from Aspergillus niger (en)
skos:prefLabel	Gene organization and molecular modeling of copper amine oxidase from Aspergillus niger Gene organization and molecular modeling of copper amine oxidase from Aspergillus niger (en)
skos:notation	RIV/61989592:15410/03:00001356!RIV/2004/MSM/154104/N
http://linked.open.../vavai/riv/strany	114
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 153100013)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Peč, Pavel Šebela, Marek Frébort, Ivo
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Pedagogická fakulta
http://linked.open...dnocenehoVysledku	608053
http://linked.open...ai/riv/idVysledku	RIV/61989592:15410/03:00001356
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	amine oxidase, Aspergillus niger, gene organization, molecular modelling, primary sequence (en)
http://linked.open.../riv/klicoveSlovo	Aspergillus niger amine oxidase gene organization molecular modelling primary sequence
http://linked.open...ontrolniKodProRIV	[3B34519E95FA]
http://linked.open...v/mistoKonaniAkce	Rome
http://linked.open...i/riv/mistoVydani	Heidelberg
http://linked.open...i/riv/nazevZdroje	8th International Congress on Amino Acids and Proteins
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Šebela, Marek Frébort, Ivo Peč, Pavel Adachi, Osao Hirota, Shun Kumagai, Hidehiko Tamaki, Hisanori
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20153100013
issn	0939-4451
number of pages	94 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Springer-Verlag
http://localhost/t...ganizacniJednotka	15410

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