About: Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Traditional plant breeding relies upon crosses and subsequent selection of genotypes to meet desirable traits. The incorporation of marker-assisted selection (MAS) into breeding strategies would result in a reduction in the number of offspring to be propagated, selected and tested. In the case of pea (Pisum sativum L.), the testing of resistance to viral pathogens such as Pea seed-borne mosaic virus (PSbMV) is included in the breeding process. Resistance to the common strains of PSbMV is conferred by a single recessive gene (eIF4E), localized on LG VI (sbm-1 locus). We have analyzed for variation in the eIF4E genomic sequences from 43 pea varieties and breeding lines, reported as donors of resistance. This enabled a comprehensive investigation of the eIF4E gene structure and mutations responsible for PSbMV resistance were identified. Subsequently, PCR-based and gene-specific single nucleotide polymorphism (SNP) and co-dominant amplicon length polymorphism markers were developed. Traditional plant breeding relies upon crosses and subsequent selection of genotypes to meet desirable traits. The incorporation of marker-assisted selection (MAS) into breeding strategies would result in a reduction in the number of offspring to be propagated, selected and tested. In the case of pea (Pisum sativum L.), the testing of resistance to viral pathogens such as Pea seed-borne mosaic virus (PSbMV) is included in the breeding process. Resistance to the common strains of PSbMV is conferred by a single recessive gene (eIF4E), localized on LG VI (sbm-1 locus). We have analyzed for variation in the eIF4E genomic sequences from 43 pea varieties and breeding lines, reported as donors of resistance. This enabled a comprehensive investigation of the eIF4E gene structure and mutations responsible for PSbMV resistance were identified. Subsequently, PCR-based and gene-specific single nucleotide polymorphism (SNP) and co-dominant amplicon length polymorphism markers were developed. (en)
Title	Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus (en)
skos:prefLabel	Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus (en)
skos:notation	RIV/61989592:15310/10:10211974!RIV11-MZE-15310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(LA08011), P(QI91A229), S, Z(MSM2678424601)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Navrátil, Milan Šafářová, Dana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	269691
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/10:10211974
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	virusresistance; sbm-1; Pea seed-borne mosaic virus; pea; Potyvirus; marker assisted breeding; eIF4E (en)
http://linked.open.../riv/klicoveSlovo	eIF4E pea Pea seed-borne mosaic virus Potyvirus marker assisted breeding sbm-1 virusresistance
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[7A828A755971]
http://linked.open...i/riv/nazevZdroje	Molecular Breeding
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Technology Transfer Platform for breeding and growing of Grain legumes. Application of conventional and molecular genetic approaches for the development of grain legumes resistant to viral and fungal pathogens and insect pests.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Navrátil, Milan Smýkal, Petr Šafářová, Dana
http://linked.open...ain/vavai/riv/wos	000282426600005
http://linked.open...n/vavai/riv/zamer	Study and utilization of biotechnological and molecular methods in genetic and breeding research of genus Pisum and Linum
issn	1380-3743
number of pages	14 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus Marker assisted pea breeding: eIF4E allele specific markers to Pea seed-borne mosaic virus

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