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  • Inosine triphosphate pyrophosphohydrolase (ITPase) catalyzing the pyrophosphohydrolysis of inosine triphosphate, deoxyinosine triphosphate and xanthosine triphosphate is involved in the metabolism and tolerance of thiopurine drugs. ITPase activity plays an important role in the prediction of toxicity to thiopurine therapy. Activities in dry blood spots were compared with fresh erythrocytes. Samples were incubated with inosine triphosphate, then inosine monophosphate was determined by a capillary electrophoresis method. Calculated enzyme activities obtained from dry blood spots were in good accordance with activity in fresh erythrocytes.
  • Inosine triphosphate pyrophosphohydrolase (ITPase) catalyzing the pyrophosphohydrolysis of inosine triphosphate, deoxyinosine triphosphate and xanthosine triphosphate is involved in the metabolism and tolerance of thiopurine drugs. ITPase activity plays an important role in the prediction of toxicity to thiopurine therapy. Activities in dry blood spots were compared with fresh erythrocytes. Samples were incubated with inosine triphosphate, then inosine monophosphate was determined by a capillary electrophoresis method. Calculated enzyme activities obtained from dry blood spots were in good accordance with activity in fresh erythrocytes. (en)
Title
  • ITPase activity in dry blood spots is comparable with that in fresh erythrocytes
  • ITPase activity in dry blood spots is comparable with that in fresh erythrocytes (en)
skos:prefLabel
  • ITPase activity in dry blood spots is comparable with that in fresh erythrocytes
  • ITPase activity in dry blood spots is comparable with that in fresh erythrocytes (en)
skos:notation
  • RIV/61989592:15110/08:10213685!RIV12-MSM-15110___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6198959205)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...dnocenehoVysledku
  • 373451
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15110/08:10213685
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  • dry blood spots; capillary electrophoresis; ITPase; enzyme activity (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [0D5CD68F2EFD]
http://linked.open...i/riv/nazevZdroje
  • Nucleosides Nucleotides and Nucleic Acids
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http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 27
http://linked.open...iv/tvurceVysledku
  • Adam, Tomáš
  • Friedecký, David
  • Tomková, Jana
  • Vyskočilová, Petra
http://linked.open...n/vavai/riv/zamer
issn
  • 1525-7770
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  • 15110
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