About: Z-DNA, a new in situ marker for transcription

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Z-DNA forms transiently behind the active RNA polymerases, because of the mechanical torsional stress produced during transcription. In this paper, we explore the possibility that the distribution of Z-DNA stretches signals the sites related to nuclear transcription. To localize transcription, the in situ assay for active RNA polymerases, that allows the elongation of the already initiated transcripts but no initiation of new ones (run-on experiments), was carried out in isolated nuclei of Allium cepa L. root meristems. Both nucleolar and non-nucleolar sites appeared labelled. Nucleoli were most active in transcription than the multiple non-nucleolar foci altogether. In situ immunodetection of Z-DNA provided images that were comparable to those obtained after the run-on assay, with one exception: while Z-DNA and transcription sites were scattered throughout the whole nucleus, Z-DNA also accumulated in the nuclear periphery, where no transcription foci were detected in the run-on ass... Z-DNA forms transiently behind the active RNA polymerases, because of the mechanical torsional stress produced during transcription. In this paper, we explore the possibility that the distribution of Z-DNA stretches signals the sites related to nuclear transcription. To localize transcription, the in situ assay for active RNA polymerases, that allows the elongation of the already initiated transcripts but no initiation of new ones (run-on experiments), was carried out in isolated nuclei of Allium cepa L. root meristems. Both nucleolar and non-nucleolar sites appeared labelled. Nucleoli were most active in transcription than the multiple non-nucleolar foci altogether. In situ immunodetection of Z-DNA provided images that were comparable to those obtained after the run-on assay, with one exception: while Z-DNA and transcription sites were scattered throughout the whole nucleus, Z-DNA also accumulated in the nuclear periphery, where no transcription foci were detected in the run-on ass... (en) Z-DNA, a new in situ marker for transcription. (cs)
Title	Z-DNA, a new in situ marker for transcription Z-DNA, a new in situ marker for transcription (en) Z-DNA, a new in situ marker for transcription (cs)
skos:prefLabel	Z-DNA, a new in situ marker for transcription Z-DNA, a new in situ marker for transcription (en) Z-DNA, a new in situ marker for transcription (cs)
skos:notation	RIV/61389030:_____/04:00109161!RIV/2005/AV0/A56005/N
http://linked.open.../vavai/riv/strany	49;55
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IBS5038351), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Černá, Adriana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	595782
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/04:00109161
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Allium cepa; Z-DNA immunodetection; in situ run-on transcription (en)
http://linked.open.../riv/klicoveSlovo	Allium cepa Z-DNA immunodetection in situ run-on transcription
http://linked.open...odStatuVydavatele	IT - Italská republika
http://linked.open...ontrolniKodProRIV	[0BFCF049B081]
http://linked.open...i/riv/nazevZdroje	EUROPEAN JOURNAL OF HISTOCHEMISTRY
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Immunodiagnostics of natural hormonal substances
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Jouve, N. Černá, Adriana De la Torre, C. Cuadrado, A. de la Espina, S. M. D.
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	1121-760X
number of pages	7 (xsd:int)

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