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  • Magnetic bead cellulose (MBC) was prepared using sol–gel transition of viscose in the presence of maghemite (γ-Fe2O3) nanoparticles. The MBC particles were then activated with p-toluenesulfonyl chloride to yield tosyl-activated magnetic bead cellulose (MBC-Ts). The microspheres were characterized by light and electron microscopy, elemental analysis and atomic absorption spectroscopy to determine morphology, size, polydispersity and content of iron and tosyl groups. The functionality of the MBC-Ts microspheres was demonstrated using proximity ligation assay (PLA) to detect vascular endothelial growth factor in femtomolar concentration range. The MBC-Ts microspheres performed equally well as commercially available microparticles that are routinely used as solid support in solid phase PLA.
  • Magnetic bead cellulose (MBC) was prepared using sol–gel transition of viscose in the presence of maghemite (γ-Fe2O3) nanoparticles. The MBC particles were then activated with p-toluenesulfonyl chloride to yield tosyl-activated magnetic bead cellulose (MBC-Ts). The microspheres were characterized by light and electron microscopy, elemental analysis and atomic absorption spectroscopy to determine morphology, size, polydispersity and content of iron and tosyl groups. The functionality of the MBC-Ts microspheres was demonstrated using proximity ligation assay (PLA) to detect vascular endothelial growth factor in femtomolar concentration range. The MBC-Ts microspheres performed equally well as commercially available microparticles that are routinely used as solid support in solid phase PLA. (en)
Title
  • A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection
  • A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection (en)
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  • A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection
  • A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection (en)
skos:notation
  • RIV/61389013:_____/13:00394938!RIV14-MSM-61389013
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
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  • I, P(7E12054), P(GAP503/10/0664)
http://linked.open...iv/cisloPeriodika
  • 3
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  • 59162
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  • RIV/61389013:_____/13:00394938
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  • bead cellulose; magnetic; protein detection (en)
http://linked.open.../riv/klicoveSlovo
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  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [E00C48C1DB08]
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  • Journal of Biotechnology
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  • 167
http://linked.open...iv/tvurceVysledku
  • Horák, Daniel
  • Yan, J.
  • Lenfeld, Jiří
  • Hammond, M.
  • Kamali-Moghaddam, M.
http://linked.open...ain/vavai/riv/wos
  • 000324040300005
issn
  • 0168-1656
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.jbiotec.2013.06.010
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