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Description
| - Aeruginosin-865 (Aer-865), isolated from terrestrial cyanobacterium Nostoc sp. Lukesova 30/93, is the first aeruginosin-type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one- and two-dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: D-Hpla, D-Leu, 5-OH-Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL-8 and ICAM-1 in hTNF-alpha-stimulated HLMVECs. Aer-865 showed significant reduction of both: with EC50 values of (3.51.5) mugmL(-1) ((4.01.7) muM) and (50.013.4) mugmL(-1) ((57.815.5) muM), respectively. Confocal laser scanning microscopy revealed that the anti-inflammatory effect of Aer-865 was directly associated with inhibition of NF-kappaB translocation to the nucleus. Moreover, Aer-865 did not show any cytotoxic effect
- Aeruginosin-865 (Aer-865), isolated from terrestrial cyanobacterium Nostoc sp. Lukesova 30/93, is the first aeruginosin-type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one- and two-dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: D-Hpla, D-Leu, 5-OH-Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL-8 and ICAM-1 in hTNF-alpha-stimulated HLMVECs. Aer-865 showed significant reduction of both: with EC50 values of (3.51.5) mugmL(-1) ((4.01.7) muM) and (50.013.4) mugmL(-1) ((57.815.5) muM), respectively. Confocal laser scanning microscopy revealed that the anti-inflammatory effect of Aer-865 was directly associated with inhibition of NF-kappaB translocation to the nucleus. Moreover, Aer-865 did not show any cytotoxic effect (en)
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Title
| - Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent
- Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent (en)
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skos:prefLabel
| - Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent
- Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent (en)
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skos:notation
| - RIV/61388971:_____/13:00424616!RIV14-MSM-61388971
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http://linked.open...avai/predkladatel
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - I, P(ED2.1.00/03.0110), P(LH11129), V
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/13:00424616
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - NMR spectroscopy; anti-inflammatory; cyanobacteria (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Hrouzek, Pavel
- Kopecký, Jiří
- Kuzma, Marek
- Novák, Petr
- Jokela, J.
- Bártová, Simona
- Hundsberger, H.
- Eger, A.
- Kapuscik, Alexandra
- Pfluger, M.
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://bibframe.org/vocab/doi
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