About: Substrate binding activates the designed triple mutant of the colicin E7 metallonuclease

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About: Substrate binding activates the designed triple mutant of the colicin E7 metallonuclease Goto Sponge Distinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The nuclease domain of colicin E7 (NColE7) cleaves DNA nonspecifically. The active center is a Zn2+-containing HNH motif at the C-terminus. The N-terminal loop is essential for the catalytic activity providing opportunity for allosteric modulation of the enzyme. To identify the key residues responsible for the structural integrity of NColE7, a virtual alanine scan was performed on a semiempirical quantum chemical level within the 25 residue long N-terminal sequence (446-470). Based on the calculations the T454A/K458A/W464A-NColE7 triple mutant (TKW) was expressed and purified. According to the agarose gel electrophoresis experiments and linear dichroism spectra the catalytic activity of the TKW mutant decreased in comparison with wild-type NColE7. The distorted structure and weakened Zn2+ binding may account for this as revealed by circular dichroism spectra, mass spectrometry, fluorescence-based thermal analysis and isothermal microcalorimetric titrations. Remarkably, the substrate induced the folding of the mutant protein. The nuclease domain of colicin E7 (NColE7) cleaves DNA nonspecifically. The active center is a Zn2+-containing HNH motif at the C-terminus. The N-terminal loop is essential for the catalytic activity providing opportunity for allosteric modulation of the enzyme. To identify the key residues responsible for the structural integrity of NColE7, a virtual alanine scan was performed on a semiempirical quantum chemical level within the 25 residue long N-terminal sequence (446-470). Based on the calculations the T454A/K458A/W464A-NColE7 triple mutant (TKW) was expressed and purified. According to the agarose gel electrophoresis experiments and linear dichroism spectra the catalytic activity of the TKW mutant decreased in comparison with wild-type NColE7. The distorted structure and weakened Zn2+ binding may account for this as revealed by circular dichroism spectra, mass spectrometry, fluorescence-based thermal analysis and isothermal microcalorimetric titrations. Remarkably, the substrate induced the folding of the mutant protein. (en)
Title	Substrate binding activates the designed triple mutant of the colicin E7 metallonuclease Substrate binding activates the designed triple mutant of the colicin E7 metallonuclease (en)
skos:prefLabel	Substrate binding activates the designed triple mutant of the colicin E7 metallonuclease Substrate binding activates the designed triple mutant of the colicin E7 metallonuclease (en)
skos:notation	RIV/61388963:_____/14:00436908!RIV15-AV0-61388963
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Kožíšek, Milan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	48476
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/14:00436908
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	binding affinity; calorimetry; zinc nuclease; substrate induced folding; protein engineering (en)
http://linked.open.../riv/klicoveSlovo	calorimetry substrate induced folding zinc nuclease binding affinity protein engineering
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[2A24739589B9]
http://linked.open...i/riv/nazevZdroje	Journal of Biological Inorganic Chemistry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Kožíšek, Milan Christensen, H. E. M. Gyurcsik, B. Nagata, K. Németh, E. Körtvélyesi, T. Thulstrup, P. W. Asaka, M. N.
http://linked.open...ain/vavai/riv/wos	000345403900005
issn	0949-8257
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00775-014-1186-6

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