Site-directed mutagenesis was used to design new trypsin variants which are catalytic active for the synthesis of peptide bonds mediated by substrate mimetics of the 4-guanidinophenyl ester type, but inactive for their cleavage.
Site-directed mutagenesis was used to design new trypsin variants which are catalytic active for the synthesis of peptide bonds mediated by substrate mimetics of the 4-guanidinophenyl ester type, but inactive for their cleavage. (en)