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  • Arrested or limited fermentation process is a widespread method used for production of alcohol-free beer (AFB). However, it usually leads to worty off-flavour and a lack of pleasant fruity flavour/aroma. The aim of this study was to isolate spontaneous mutants of Saccharomyces pastorianus strain 2 resistant to 5,5,5-trifluoro-DL-leucine, since the resistance is related to overproduction of flavour active isoamyl alcohol (IAAL) and isoamyl acetate (IAAC). The mutants were the subject of selection during series of screening tests aimed at selecting the best producer of target compounds (IAAL and IAAC). Subsequently, the overproduction stability of the selected mutant strain was verified in four consecutive fermentations of AFBs. A higher pitching rate and increased fermentation temperature did not result in an overall improvement in the production of the target compounds. Sensory analysis proved the flavouring effect of the spontaneous lager yeast mutant on the AFB produced by arrested fermentation. Copyright ? 2013 The Institute of Brewing & Distilling
  • Arrested or limited fermentation process is a widespread method used for production of alcohol-free beer (AFB). However, it usually leads to worty off-flavour and a lack of pleasant fruity flavour/aroma. The aim of this study was to isolate spontaneous mutants of Saccharomyces pastorianus strain 2 resistant to 5,5,5-trifluoro-DL-leucine, since the resistance is related to overproduction of flavour active isoamyl alcohol (IAAL) and isoamyl acetate (IAAC). The mutants were the subject of selection during series of screening tests aimed at selecting the best producer of target compounds (IAAL and IAAC). Subsequently, the overproduction stability of the selected mutant strain was verified in four consecutive fermentations of AFBs. A higher pitching rate and increased fermentation temperature did not result in an overall improvement in the production of the target compounds. Sensory analysis proved the flavouring effect of the spontaneous lager yeast mutant on the AFB produced by arrested fermentation. Copyright ? 2013 The Institute of Brewing & Distilling (en)
Title
  • Production of alcohol-free beer with elevated amounts of flavouring compounds using lager yeast mutants
  • Production of alcohol-free beer with elevated amounts of flavouring compounds using lager yeast mutants (en)
skos:prefLabel
  • Production of alcohol-free beer with elevated amounts of flavouring compounds using lager yeast mutants
  • Production of alcohol-free beer with elevated amounts of flavouring compounds using lager yeast mutants (en)
skos:notation
  • RIV/60461373:22330/13:43895122!RIV14-MSM-22330___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
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  • Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 100001
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/13:43895122
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • 5,5,5-trifluoro-DL-leucine; isoamyl alcohol; isoamyl acetate; yeast; mutation; alcohol-free beer (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [8CEC5088A5F5]
http://linked.open...i/riv/nazevZdroje
  • Journal of the Institute of Brewing
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 119
http://linked.open...iv/tvurceVysledku
  • Brányik, Tomáš
  • Karabín, Marcel
  • Siříšťová, Lucie
  • Strejc, Jan
  • Silva, Joao B. Almeida
http://linked.open...ain/vavai/riv/wos
  • 000327665800010
http://linked.open...n/vavai/riv/zamer
issn
  • 0046-9750
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/jib.72
http://localhost/t...ganizacniJednotka
  • 22330
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