About: Towards proteomic analysis of milk proteins in historical building materials

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The addition of proteinaceous binders to mortars and plasters has a long tradition. The protein additions were identified in many sacral and secular historical buildings. For this method of peptide mass mapping, three model mortar samples with protein additives were prepared. These samples were analysed fresh (1-2 weeks old) and after 9 months of natural ageing. The optimal duration of tryptic cleavage (2 h) and the lowest amount of material needed for relevant analysis of fresh and weathered samples were found: the sufficient amounts of weathered and fresh mortars were set to 0.05 and 0.005 g. The list of main tryptic peptides coming from milk additives (bovine milk, curd, and whey), their relative intensities and theoretical amino acid sequences assignment is presented. Several sequences have been %22de novo%22 confirmed by mass spectrometry. The addition of proteinaceous binders to mortars and plasters has a long tradition. The protein additions were identified in many sacral and secular historical buildings. For this method of peptide mass mapping, three model mortar samples with protein additives were prepared. These samples were analysed fresh (1-2 weeks old) and after 9 months of natural ageing. The optimal duration of tryptic cleavage (2 h) and the lowest amount of material needed for relevant analysis of fresh and weathered samples were found: the sufficient amounts of weathered and fresh mortars were set to 0.05 and 0.005 g. The list of main tryptic peptides coming from milk additives (bovine milk, curd, and whey), their relative intensities and theoretical amino acid sequences assignment is presented. Several sequences have been %22de novo%22 confirmed by mass spectrometry. (en)
Title	Towards proteomic analysis of milk proteins in historical building materials Towards proteomic analysis of milk proteins in historical building materials (en)
skos:prefLabel	Towards proteomic analysis of milk proteins in historical building materials Towards proteomic analysis of milk proteins in historical building materials (en)
skos:notation	RIV/60461373:22330/09:00022152!RIV10-MSM-22330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP203/07/P360), Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	1-3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hynek, Radovan Kučková, Štěpánka Kodíček, Milan Crhová, Michaela Vaňková, Lucie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	346579
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/09:00022152
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Peptide mass mapping; Database; MALDI-TOF MS; Model mortar sample; Protein additive (en)
http://linked.open.../riv/klicoveSlovo	Protein additive Database Peptide mass mapping Model mortar sample MALDI-TOF MS
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[151C9403ABB8]
http://linked.open...i/riv/nazevZdroje	International Journal of Mass Spectrometry
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Characterisation and identification of proteinaceous binders in art works by MALDI-TOF mass spectrometry
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	284
http://linked.open...iv/tvurceVysledku	Crhová, Michaela Hynek, Radovan Kodíček, Milan Kučková, Štěpánka Vaňková, Lucie Hnízda, Aleš
http://linked.open...ain/vavai/riv/wos	000268059100006
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
issn	1387-3806
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	22330
is http://linked.open...avai/riv/vysledek of	Towards proteomic analysis of milk proteins in historical building materials Towards proteomic analysis of milk proteins in historical building materials Towards proteomic analysis of milk proteins in historical building materials Towards proteomic analysis of milk proteins in historical building materials

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